TY - JOUR
T1 - Automated algorithms for multiscale morphometry of neuronal dendrites
AU - Weaver, Christina M.
AU - Hof, Patrick R.
AU - Wearne, Susan L.
AU - Lindquist, W. Brent
PY - 2004/7
Y1 - 2004/7
N2 - We describe the synthesis of automated neuron branching morphology and spine detection algorithms to provide multiscale three-dimensional morphological analysis of neurons. The resulting software is applied to the analysis of a high-resolution (0.098 μm × 0.098 μm × 0.081 μm) image of an entire pyramidal neuron from layer III of the superior temporal cortex in rhesus macaque monkey. The approach provides a highly automated, complete morphological analysis of the entire neuron; each dendritic branch segment is characterized by several parameters, including branch order, length, and radius as a function of distance along the branch, as well as by the locations, lengths, shape classification (e.g., mushroom, stubby, thin), and density distribution of spines on the branch. Results for this automated analysis are compared to published results obtained by other computer-assisted manual means.
AB - We describe the synthesis of automated neuron branching morphology and spine detection algorithms to provide multiscale three-dimensional morphological analysis of neurons. The resulting software is applied to the analysis of a high-resolution (0.098 μm × 0.098 μm × 0.081 μm) image of an entire pyramidal neuron from layer III of the superior temporal cortex in rhesus macaque monkey. The approach provides a highly automated, complete morphological analysis of the entire neuron; each dendritic branch segment is characterized by several parameters, including branch order, length, and radius as a function of distance along the branch, as well as by the locations, lengths, shape classification (e.g., mushroom, stubby, thin), and density distribution of spines on the branch. Results for this automated analysis are compared to published results obtained by other computer-assisted manual means.
UR - http://www.scopus.com/inward/record.url?scp=2942593692&partnerID=8YFLogxK
U2 - 10.1162/089976604323057425
DO - 10.1162/089976604323057425
M3 - Article
C2 - 15165394
AN - SCOPUS:2942593692
SN - 0899-7667
VL - 16
SP - 1353
EP - 1383
JO - Neural Computation
JF - Neural Computation
IS - 7
ER -