Abstract The effect of an atherogenic diet on hepatic cytokine production in relation to aortic lesion formation was assessed in LDL receptor null (LDLr-/-) mice. Mice (n=11/group) were fed an atherogenic (20% fat, 0.2% cholesterol, w/w) or control (4% fat, 0.02% cholesterol, w/w) diet for 32 weeks. Cytokine concentrations and tissue lipid content were measured by ELISA and enzymatic methods, respectively. The atherogenic diet, relative to the control diet, significantly increased the hepatic production of interleukin-6 (IL-6, 1.4-fold, P<0.05) and tumor necrosis factor alpha (TNF, 2.0-fold, P<0.05). The atherogenic diet, relative to the control diet, also resulted in significantly higher content of liver triglyceride (3.5-fold, P<0.01), total cholesterol (4.2-fold, P<0.01) and esterified cholesterol (7.9-fold, P<0.001). Greater accumulation of aortic esterified cholesterol (6.4-fold, P<0.001) and higher plasma concentrations of TNF (4.4-fold, P<0.01) and IL-6 (2.9-fold, P<0.001) w
|State||Published - Mar 2010|
Wang, S., Wu, Y. C., Wu, D., Matthan, N., Lamon-Fava, S., Srinivasan, I., Reed, D., & Lichtenstein, A. (2010, Mar). Atherogenic Diet Promotes Liver Inflammation, Fatty Liver Formation & Atherogenesis in LDL Receptor Null Mice. FASEB J.