TY - GEN
T1 - Atherogenic Diet Promotes Liver Inflammation, Fatty Liver Formation & Atherogenesis in LDL Receptor Null Mice
AU - Wang, Shu
AU - Wu, Yi Chieh
AU - Wu, Dayong
AU - Matthan, Nirupa
AU - Lamon-Fava, Stefania
AU - Srinivasan, Iswarya
AU - Reed, Debra
AU - Lichtenstein, Alice
PY - 2010/3
Y1 - 2010/3
N2 - Abstract
The effect of an atherogenic diet on hepatic cytokine production in relation to aortic lesion formation was assessed in LDL receptor null (LDLr-/-) mice. Mice (n=11/group) were fed an atherogenic (20% fat, 0.2% cholesterol, w/w) or control (4% fat, 0.02% cholesterol, w/w) diet for 32 weeks. Cytokine concentrations and tissue lipid content were measured by ELISA and enzymatic methods, respectively. The atherogenic diet, relative to the control diet, significantly increased the hepatic production of interleukin-6 (IL-6, 1.4-fold, P<0.05) and tumor necrosis factor alpha (TNF, 2.0-fold, P<0.05). The atherogenic diet, relative to the control diet, also resulted in significantly higher content of liver triglyceride (3.5-fold, P<0.01), total cholesterol (4.2-fold, P<0.01) and esterified cholesterol (7.9-fold, P<0.001). Greater accumulation of aortic esterified cholesterol (6.4-fold, P<0.001) and higher plasma concentrations of TNF (4.4-fold, P<0.01) and IL-6 (2.9-fold, P<0.001) w
AB - Abstract
The effect of an atherogenic diet on hepatic cytokine production in relation to aortic lesion formation was assessed in LDL receptor null (LDLr-/-) mice. Mice (n=11/group) were fed an atherogenic (20% fat, 0.2% cholesterol, w/w) or control (4% fat, 0.02% cholesterol, w/w) diet for 32 weeks. Cytokine concentrations and tissue lipid content were measured by ELISA and enzymatic methods, respectively. The atherogenic diet, relative to the control diet, significantly increased the hepatic production of interleukin-6 (IL-6, 1.4-fold, P<0.05) and tumor necrosis factor alpha (TNF, 2.0-fold, P<0.05). The atherogenic diet, relative to the control diet, also resulted in significantly higher content of liver triglyceride (3.5-fold, P<0.01), total cholesterol (4.2-fold, P<0.01) and esterified cholesterol (7.9-fold, P<0.001). Greater accumulation of aortic esterified cholesterol (6.4-fold, P<0.001) and higher plasma concentrations of TNF (4.4-fold, P<0.01) and IL-6 (2.9-fold, P<0.001) w
M3 - Other contribution
VL - 24
PB - FASEB J.
ER -