TY - JOUR
T1 - AtCHIP, a U-Box-containing E3 ubiquitin ligase, plays a critical role in temperature stress tolerance in Arabidopsis
AU - Yan, Juqiang
AU - Wang, Jing
AU - Li, Qingtian
AU - Hwang, Jae Ryoung
AU - Patterson, Cam
AU - Zhang, Hong
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/6/1
Y1 - 2003/6/1
N2 - The Arabidopsis gene AtCHIP encodes a protein with three tetratricopeptide repeats and a U-box domain, which is structurally similar to the animal CHIP proteins, a new class of E3 ubiquitin ligases. Like animal CHIP proteins, AtCHIP has E3 ubiquitin ligase activity in vitro. AtCHIP is a single-copy gene, and its transcript is up-regulated by several stress conditions such as low and high temperatures. However, increased AtCHIP expression alone was not correlated with increased stress tolerance; in fact, overexpression of AtCHIP in Arabidopsis rendered plants more sensitive to both low- and high-temperature treatments. Higher electrolyte leakage was observed in leaves of AtCHIP overexpression plants after chilling temperature treatment, suggesting that membrane function is likely impaired in these plants under such a condition. These results indicate that AtCHIP plays an important role in plant cellular metabolism under temperature stress conditions.
AB - The Arabidopsis gene AtCHIP encodes a protein with three tetratricopeptide repeats and a U-box domain, which is structurally similar to the animal CHIP proteins, a new class of E3 ubiquitin ligases. Like animal CHIP proteins, AtCHIP has E3 ubiquitin ligase activity in vitro. AtCHIP is a single-copy gene, and its transcript is up-regulated by several stress conditions such as low and high temperatures. However, increased AtCHIP expression alone was not correlated with increased stress tolerance; in fact, overexpression of AtCHIP in Arabidopsis rendered plants more sensitive to both low- and high-temperature treatments. Higher electrolyte leakage was observed in leaves of AtCHIP overexpression plants after chilling temperature treatment, suggesting that membrane function is likely impaired in these plants under such a condition. These results indicate that AtCHIP plays an important role in plant cellular metabolism under temperature stress conditions.
UR - http://www.scopus.com/inward/record.url?scp=0038796900&partnerID=8YFLogxK
U2 - 10.1104/pp.103.020800
DO - 10.1104/pp.103.020800
M3 - Article
C2 - 12805616
AN - SCOPUS:0038796900
VL - 132
SP - 861
EP - 869
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 2
ER -