Abstract
Aims: To design and synthesize novel N-(1-phenyl-2,3-dihydroxypropyl) arachidonylamides and evaluate their analgesic and anti-inflammatory potential. Main methods: The murine macrophage cell line RAW 264.7 has been widely used as a model for inflammatory responses in vitro. Our model consists of cultured monolayers of RAW 264.7 cells in which media concentrations of 15-deoxy-Δ13,14-PGJ2 (PGJ) are measured by ELISA following LPS (10 ng/ml) stimulation and treatment with 0.1, 0.3, 1.0, 3.0 and 10 μM concentrations of the compounds. Key findings: Our data indicate that several of our compounds have the capacity to increase production of PGJ and may also increase the occurrence of programmed cell death (apoptosis). Significance: Thus these agents are potential candidates for the therapy of conditions characterized by ongoing (chronic) inflammation and its associated pain.
Original language | English |
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Pages (from-to) | 506-511 |
Number of pages | 6 |
Journal | Life Sciences |
Volume | 92 |
Issue number | 8-9 |
DOIs | |
State | Published - Mar 19 2013 |
Keywords
- 15-Deoxy-Δ -PGJ (PGJ) stimulation
- Anandamide
- Apoptosis
- Arachidonyl amide
- Cannabinoid receptor