Asymmetric synthesis of novel N-(1-phenyl-2,3-dihydroxypropyl) arachidonylamides and evaluation of their anti-inflammatory activity

Padmanabha V. Kattamuri, Rebecca Salmonsen, Catherine McQuain, Sumner Burstein, Hao Sun, Guigen Li

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Aims: To design and synthesize novel N-(1-phenyl-2,3-dihydroxypropyl) arachidonylamides and evaluate their analgesic and anti-inflammatory potential. Main methods: The murine macrophage cell line RAW 264.7 has been widely used as a model for inflammatory responses in vitro. Our model consists of cultured monolayers of RAW 264.7 cells in which media concentrations of 15-deoxy-Δ13,14-PGJ2 (PGJ) are measured by ELISA following LPS (10 ng/ml) stimulation and treatment with 0.1, 0.3, 1.0, 3.0 and 10 μM concentrations of the compounds. Key findings: Our data indicate that several of our compounds have the capacity to increase production of PGJ and may also increase the occurrence of programmed cell death (apoptosis). Significance: Thus these agents are potential candidates for the therapy of conditions characterized by ongoing (chronic) inflammation and its associated pain.

Original languageEnglish
Pages (from-to)506-511
Number of pages6
JournalLife Sciences
Volume92
Issue number8-9
DOIs
StatePublished - Mar 19 2013

Keywords

  • 15-Deoxy-Δ -PGJ (PGJ) stimulation
  • Anandamide
  • Apoptosis
  • Arachidonyl amide
  • Cannabinoid receptor

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