TY - JOUR
T1 - Association between Mitochondrial DNA Sequence Variants and VO2 max Trainability
AU - Vellers, Heather L
AU - Verhein, Kirsten C
AU - Burkholder, Adam B
AU - Lee, Jae Hoon
AU - Kim, Youngmin
AU - Lightfoot, J Timothy
AU - Shi, Min
AU - Weinberg, Clarice R
AU - Sarzynski, Mark A
AU - Bouchard, Claude
AU - Kleeberger, Steven R
PY - 2020/10
Y1 - 2020/10
N2 - PURPOSE: We designed the study to determine whether mitochondrial DNA (mtDNA) haplogroup, sequence, and heteroplasmy differed between individuals previously characterized as low- (LR) or high-responders (HR) as defined by their VO2 max response to a standardized aerobic exercise training program. METHODS: DNA was isolated from whole blood in subjects from the HERITAGE Family Study that were determined to be either HR (n=15) or LR (n=15). mtDNA was amplified by long-range polymerase chain reaction, then tagged with Nextera libraries and sequenced on a MiSeq instrument. RESULTS: Different mtDNA haplogroup subtypes were found in HR and LR individuals. Compared to HR subjects, significantly more LR subjects had variants in 13 sites, including seven in hypervariable (HV) regions: HV2 (G185A: 0 vs 6, p = 0.02; G228A: 0 vs 5, p = 0.04; C295T: 0 vs 6; p = 0.04), HV3 (C462T: 0 vs 5, p = 0.04; T489C: 0 vs 5; p = 0.04), and HV1 (C16068T: 0 vs 6, p = 0.02; T16125C: 0 vs 6, p = 0.02). R
AB - PURPOSE: We designed the study to determine whether mitochondrial DNA (mtDNA) haplogroup, sequence, and heteroplasmy differed between individuals previously characterized as low- (LR) or high-responders (HR) as defined by their VO2 max response to a standardized aerobic exercise training program. METHODS: DNA was isolated from whole blood in subjects from the HERITAGE Family Study that were determined to be either HR (n=15) or LR (n=15). mtDNA was amplified by long-range polymerase chain reaction, then tagged with Nextera libraries and sequenced on a MiSeq instrument. RESULTS: Different mtDNA haplogroup subtypes were found in HR and LR individuals. Compared to HR subjects, significantly more LR subjects had variants in 13 sites, including seven in hypervariable (HV) regions: HV2 (G185A: 0 vs 6, p = 0.02; G228A: 0 vs 5, p = 0.04; C295T: 0 vs 6; p = 0.04), HV3 (C462T: 0 vs 5, p = 0.04; T489C: 0 vs 5; p = 0.04), and HV1 (C16068T: 0 vs 6, p = 0.02; T16125C: 0 vs 6, p = 0.02). R
M3 - Article
SP - 2303
EP - 2309
JO - Medicine & Science in Sports & Exercise
JF - Medicine & Science in Sports & Exercise
ER -