TY - JOUR
T1 - APSR1, a novel gene required for meristem maintenance, is negatively regulated by low phosphate availability
AU - González-Mendoza, Víctor
AU - Zurita-Silva, Andrés
AU - Sánchez-Calderón, Lenin
AU - Sánchez-Sandoval, María Eugenia
AU - Oropeza-Aburto, Araceli
AU - Gutiérrez-Alanís, Dolores
AU - Alatorre-Cobos, Fulgencio
AU - Herrera-Estrella, Luis
N1 - Funding Information:
We thank Hongbin Zhang and The Department of Soil and Crop Sciences from Texas A&M University for providing the BIBAC clones. V.M.G_M. is indebted to CONACYT (Mexico) for a PhD fellowship. This work was supported in part by grants from the Howard Hughes Medical Institute (grant 55005946 ) and CONACyT (grant 299/43979 ) to L.H.-E.
PY - 2013/5
Y1 - 2013/5
N2 - Proper root growth is crucial for anchorage, exploration, and exploitation of the soil substrate. Root growth is highly sensitive to a variety of environmental cues, among them water and nutrient availability have a great impact on root development. Phosphorus (P) availability is one of the most limiting nutrients that affect plant growth and development under natural and agricultural environments. Root growth in the direction of the long axis proceeds from the root tip and requires the coordinated activities of cell proliferation, cell elongation and cell differentiation. Here we report a novel gene, APSR1 (Altered Phosphate Starvation Response1), involved in root meristem maintenance. The loss of function mutant apsr1-1 showed a reduction in primary root length and root apical meristem size, short differentiated epidermal cells and long root hairs. Expression of APSR1 gene decreases in response to phosphate starvation and apsr1-1 did not show the typical progressive decrease of undifferentiated cells at root tip when grown under P limiting conditions. Interestingly, APSR1 expression pattern overlaps with root zones of auxin accumulation. Furthermore, apsr1-1 showed a clear decrease in the level of the auxin transporter PIN7. These data suggest that APSR1 is required for the coordination of cell processes necessary for correct root growth in response to phosphate starvation conceivably by direct or indirect modulation of PIN7. We also propose, based on its nuclear localization and structure, that APSR1 may potentially be a member of a novel group of transcription factors.
AB - Proper root growth is crucial for anchorage, exploration, and exploitation of the soil substrate. Root growth is highly sensitive to a variety of environmental cues, among them water and nutrient availability have a great impact on root development. Phosphorus (P) availability is one of the most limiting nutrients that affect plant growth and development under natural and agricultural environments. Root growth in the direction of the long axis proceeds from the root tip and requires the coordinated activities of cell proliferation, cell elongation and cell differentiation. Here we report a novel gene, APSR1 (Altered Phosphate Starvation Response1), involved in root meristem maintenance. The loss of function mutant apsr1-1 showed a reduction in primary root length and root apical meristem size, short differentiated epidermal cells and long root hairs. Expression of APSR1 gene decreases in response to phosphate starvation and apsr1-1 did not show the typical progressive decrease of undifferentiated cells at root tip when grown under P limiting conditions. Interestingly, APSR1 expression pattern overlaps with root zones of auxin accumulation. Furthermore, apsr1-1 showed a clear decrease in the level of the auxin transporter PIN7. These data suggest that APSR1 is required for the coordination of cell processes necessary for correct root growth in response to phosphate starvation conceivably by direct or indirect modulation of PIN7. We also propose, based on its nuclear localization and structure, that APSR1 may potentially be a member of a novel group of transcription factors.
KW - Arabidopsis
KW - Auxin gradients
KW - Morphological changes
KW - PIN proteins
KW - Phosphate starvation response
KW - Root growth
UR - http://www.scopus.com/inward/record.url?scp=84873562826&partnerID=8YFLogxK
U2 - 10.1016/j.plantsci.2012.12.015
DO - 10.1016/j.plantsci.2012.12.015
M3 - Article
C2 - 23498857
AN - SCOPUS:84873562826
SN - 0168-9452
VL - 205-206
SP - 2
EP - 12
JO - Plant Science
JF - Plant Science
ER -