Applications of quantitative proteomics in plant research

Mehdi Mirzaei, Yunqi Wu, David Handler, Tim Maher, Dana Pascovici, Prathiba Ravishankar, Masoud Zabet Moghaddam, Paul A. Haynes, Ghasem Hosseini Salekdeh, Joel M. Chick, Robert D. Willows

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

8 Scopus citations

Abstract

Over the past two decades, we witnessed significant technological advances in Proteomics. Methodology and instrumentation have developed remarkably and proteomics has become a priority field of research in biology. Furthermore, analysis of the entire proteome of many organisms became possible due to complete genome sequencing. The advances in mass spectrometry instrumentation and bioinformatics tools have advanced quantitative proteomics techniques, resulting in important contributions to the biological knowledge of plants. In this chapter, we highlight the recent applications of proteomics in plants, in both model and non-model species. We then discuss the pros and cons of the major quantitative approaches implemented in plant studies. Next, we describe the most studied post-translational modifications (PTMs) in plant research, and lastly, we review the challenges of bioinformatics data analysis in the plant proteomics field.

Original languageEnglish
Title of host publicationAgricultural Proteomics Volume 1
Subtitle of host publicationCrops, Horticulture, Farm Animals, Food, Insect and Microorganisms
PublisherSpringer International Publishing
Pages1-29
Number of pages29
ISBN (Electronic)9783319432755
ISBN (Print)9783319432731
DOIs
StatePublished - Jan 1 2016

Keywords

  • Mass spectrometry
  • Post-translational modification
  • Quantitative proteomics

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