Antimicrobial resistance hidden within multiserovar salmonella populations

Amy T. Siceloff, Naomi Ohta, Keri N. Norman, Guy H. Loneragan, Bo Norby, H. Morgan Scott, Nikki W. Shariat

Research output: Contribution to journalArticlepeer-review

Abstract

Salmonella enterica can exist in food animals as multiserovar populations, and different serovars can harbor diverse antimicrobial resistance (AMR) profiles. Conventional Salmonella isolation assesses AMR only in the most abundant members of a multiserovar population, which typically reflects their relative abundance in the initial sample. Therefore, AMR in underlying serovars is an undetected reservoir that can readily be expanded upon antimicrobial use. CRISPR-SeroSeq profiling demonstrated that 60% of cattle fecal samples harbored multiple serovars, including low levels of Salmonella serovar Reading in 11% of samples, which were not found by culture-based Salmonella isolation. An in vitro challenge revealed that Salmonella serovar Reading was tetracycline resistant, while more abundant serovars were susceptible. This study highlights the importance of AMR surveillance in multiserovar populations.

Original languageEnglish
Article numbere00048
JournalAntimicrobial Agents and Chemotherapy
Volume65
Issue number6
DOIs
StatePublished - Jun 2021

Keywords

  • Antimicrobial resistance
  • CRISPR-SeroSeq
  • Cattle
  • Salmonella
  • Serovar Reading
  • Tetracycline

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