TY - JOUR
T1 - Antidepressant like effects of piperine in chronic mild stress treated mice and its possible mechanisms
AU - Li, Song
AU - Wang, Che
AU - Wang, Minwei
AU - Li, Wei
AU - Matsumoto, Kinzo
AU - Tang, Yiyuan
N1 - Funding Information:
This project was funded by the National Natural Sciences Foundation of China (NSFC60472017) and partly by China Postdoctor Foundation (20060400974).
PY - 2007/3/20
Y1 - 2007/3/20
N2 - In this study, we investigated the antidepressant-like effect of piperine in mice exposed to chronic mild stress (CMS) procedure. Repeated administration of piperine for 14 days at the doses of 2.5, 5 and 10 mg/kg reversed the CMS-induced changes in sucrose consumption, plasma corticosterone level and open field activity. Furthermore, the decreased proliferation of hippocampal progenitor cells was ameliorated and the level of brain-derived neurotrophic factor (BDNF) in hippocampus of CMS stressed mice was up-regulated by piperine treatment in the same time course. In addition, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactic dehydrogenase (LDH) assays showed that piperine (6.25-25 μM) or fluoxetine (FLU, 1 μM) dose-dependently protected primary cultured hippocampal neurons from the lesion induced by 10 μM corticosterone (CORT). Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the messenger ribonucleic acid (mRNA) level of BDNF in cultured neurons. Treatment with piperine (6.25-25 μM) for 72 h reversed the CORT-induced reduction of BDNF mRNA expression in cultured hippocampal neurons. In summary, up-regulation of the progenitor cell proliferation of hippocampus and cytoprotective activity might be mechanisms involved in the antidepressant-like effect of piperine, which may be closely related to the elevation of hippocampal BDNF level.
AB - In this study, we investigated the antidepressant-like effect of piperine in mice exposed to chronic mild stress (CMS) procedure. Repeated administration of piperine for 14 days at the doses of 2.5, 5 and 10 mg/kg reversed the CMS-induced changes in sucrose consumption, plasma corticosterone level and open field activity. Furthermore, the decreased proliferation of hippocampal progenitor cells was ameliorated and the level of brain-derived neurotrophic factor (BDNF) in hippocampus of CMS stressed mice was up-regulated by piperine treatment in the same time course. In addition, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactic dehydrogenase (LDH) assays showed that piperine (6.25-25 μM) or fluoxetine (FLU, 1 μM) dose-dependently protected primary cultured hippocampal neurons from the lesion induced by 10 μM corticosterone (CORT). Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the messenger ribonucleic acid (mRNA) level of BDNF in cultured neurons. Treatment with piperine (6.25-25 μM) for 72 h reversed the CORT-induced reduction of BDNF mRNA expression in cultured hippocampal neurons. In summary, up-regulation of the progenitor cell proliferation of hippocampus and cytoprotective activity might be mechanisms involved in the antidepressant-like effect of piperine, which may be closely related to the elevation of hippocampal BDNF level.
KW - Antidepressant
KW - Brain-derived neurotrophic factor
KW - Chronic mild stress
KW - Hippocampal progenitor cell proliferation
KW - Piperine
KW - Primary cultured hippocampal neuron
UR - http://www.scopus.com/inward/record.url?scp=33847624059&partnerID=8YFLogxK
U2 - 10.1016/j.lfs.2006.12.027
DO - 10.1016/j.lfs.2006.12.027
M3 - Article
C2 - 17289085
AN - SCOPUS:33847624059
SN - 0024-3205
VL - 80
SP - 1373
EP - 1381
JO - Life Sciences
JF - Life Sciences
IS - 15
ER -