Antibiotic-resistant escherichia coli and salmonella from the feces of food animals in the east province of Rwanda

Rosine Manishimwe, Paola M. Moncada, Vestine Musanayire, Anselme Shyaka, H. Morgan Scott, Guy H. Loneragan

Research output: Contribution to journalArticlepeer-review

Abstract

In Rwanda, information on antibiotic resistance in food animals is scarce. This study was conducted to detect and phenotypically characterize antibiotic-resistant Escherichia coli and Salmonella in feces of cattle, goats, pigs, and poultry in the East province of Rwanda. We isolated non-type-specific (NTS) E. coli and Salmonella using plain culture media. In addition, we used Mac-Conkey agar media supplemented with cefotaxime at 1.0 μg/mL and ciprofloxacin at 0.5 μg/mL to increase the probability of detecting E. coli with low susceptibility to third-generation cephalospor-ins and quinolones, respectively. Antibiotic susceptibility testing was performed using the disk diffusion test. Among 540 NTS E. coli isolates, resistance to tetracycline was the most frequently ob-served (35.6%), followed by resistance to ampicillin (19.6%) and streptomycin (16.5%). Percentages of NTS E. coli resistant to all three antibiotics and percentages of multidrug-resistant strains were higher in isolates from poultry. All isolated Salmonella were susceptible to all antibiotics. The sam-ple-level prevalence for resistance to third-generation cephalosporins was estimated at 35.6% with all third-generation cephalosporin-resistant E. coli, expressing an extended-spectrum beta-lac-tamase phenotype. The sample-level prevalence for quinolone resistance was estimated at 48.3%. These results provided a baseline for future research and the development of integrated surveillance initiatives.

Original languageEnglish
Article number1013
JournalAnimals
Volume11
Issue number4
DOIs
StatePublished - Apr 2021

Keywords

  • Antibiotic resistance
  • E. coli
  • Food animals
  • Rwanda
  • Salmonella

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