TY - JOUR
T1 - Angiotensinogen gene expression in adipose tissue
T2 - Analysis of obese models and hormonal and nutritional control
AU - Jones, Brynn H.
AU - Standridge, Melissa K.
AU - Taylor, James W.
AU - Moustaïd, Naïma
PY - 1997/7
Y1 - 1997/7
N2 - Synthesis of angiotensin II (ANG II) has recently been described in adipose cells and has been linked to regulation of adiposity. Angiotensinogen (AGT), the substrate from which ANG II is formed, was previously shown to be elevated in adipose tissue of obese (ob/ob and db/db) mice and regulated by nutritional manipulation. It is unknown, however, whether overexpression of adipose AGT can be extended to other models of obesity and whether hormonal and/or nutritional factors directly regulate ACT expression in adipocytes. We investigated these possibilities by analyzing AGT mRNA levels in adipose tissue of obese Zucker rats, viable yellow (A(vy)) mice, and humans and by treating 3T3-L1 adipocytes with insulin, glucose, and a β-adrenergic agonist. We demonstrate that AGT mRNA is decreased by ~50 and 80%, respectively, in adipose tissue of obese vs. lean Zucker rats and A(vy) mice. We also report that AGT is expressed at variable levels in human adipose tissue. Finally, we show that AGT mRNA is upregulated by insulin and downregulated by β-adrenergic stimulation in adipocytes.
AB - Synthesis of angiotensin II (ANG II) has recently been described in adipose cells and has been linked to regulation of adiposity. Angiotensinogen (AGT), the substrate from which ANG II is formed, was previously shown to be elevated in adipose tissue of obese (ob/ob and db/db) mice and regulated by nutritional manipulation. It is unknown, however, whether overexpression of adipose AGT can be extended to other models of obesity and whether hormonal and/or nutritional factors directly regulate ACT expression in adipocytes. We investigated these possibilities by analyzing AGT mRNA levels in adipose tissue of obese Zucker rats, viable yellow (A(vy)) mice, and humans and by treating 3T3-L1 adipocytes with insulin, glucose, and a β-adrenergic agonist. We demonstrate that AGT mRNA is decreased by ~50 and 80%, respectively, in adipose tissue of obese vs. lean Zucker rats and A(vy) mice. We also report that AGT is expressed at variable levels in human adipose tissue. Finally, we show that AGT mRNA is upregulated by insulin and downregulated by β-adrenergic stimulation in adipocytes.
KW - 3T3-L1 adipocytes
KW - A(vy) mouse
KW - Human adipose tissue
KW - Zucker rat
UR - http://www.scopus.com/inward/record.url?scp=0030786542&partnerID=8YFLogxK
U2 - 10.1152/ajpregu.1997.273.1.r236
DO - 10.1152/ajpregu.1997.273.1.r236
M3 - Article
C2 - 9249555
AN - SCOPUS:0030786542
SN - 0363-6119
VL - 273
SP - R236-R242
JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
IS - 1 42-1
ER -