Angiotensin II increases leptin secretion by 3T3-L1 and human adipocytes via a prostaglandin-independent mechanism

Suyeon Kim, Jay Whelan, Kate Claycombe, David B. Reath, Naima Moustaid-Moussa

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

We previously reported that angiotensin II (Ang II) increases adipocyte fatty acid synthesis and triglyceride content. Triglyceride stores or adiposity correlate positively with the amount of circulating leptin. Ang II was proposed to increase adipocyte differentiation and growth by promoting prostaglandin (PG) production. The purpose of this study was to determine whether Ang II increases leptin secretion via a PG-dependent mechanism. Physiologic doses of Ang II significantly increased leptin secretion by 3T3-L1 adipocytes and human adipocytes. Elevation of PG secretions was elicited at physiologic concentrations of Ang II (P < 0.05). Secretions of 6-keto PGF, a stable derivative of PGI2, and PGE2 were induced by physiologic concentrations of Ang II in a time-responsive fashion (P < 0.05). Inhibition of PG synthesis by indomethacin and aspirin significantly suppressed basal as well as Ang II-induced PG levels, but did not significantly affect basal and Ang II-induced leptin secretion. In conclusion, although Ang II stimulates both leptin and PG secretion by adipocytes, regulation of leptin secretion by Ang II in adipocytes is not mediated by a PG-dependent mechanism.

Original languageEnglish
Pages (from-to)1135-1140
Number of pages6
JournalJournal of Nutrition
Volume132
Issue number6
DOIs
StatePublished - 2002

Keywords

  • Adipocytes
  • Angiotensin II
  • Leptin
  • Prostaglandins

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