Analysis of veterinary growth promoters in airborne particulate matter by liquid chromatography-tandem mass spectrometry

Brett R. Blackwell, M. D. Buser, B. J. Johnson, M. Baker, G. P. Cobb, P. N. Smith

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

Abstract

Veterinary growth promoters are widely used throughout the United States in livestock production. In beef production, steroid hormones are administered to an estimated 90% of cattle. These include the synthetic steroids trenbolone acetate and melengestrol acetate, as well 17ß-estradiol. Given the endocrine-modulating activity of steroid growth promoters, a sensitive and reliable analytical method is needed to detect trenbolone, melengestrol acetate, estradiol and related residues in environmental matrices. We have developed a method that incorporates solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry for he simultaneous determination of trendione, trenbolone, melengestrol acetate, estrone, and estradiol in airborne particulate matter samples. Sample preparation involved liquid-solid extraction followed by cleanup with SPE cartridges. Analytes were separated using reversed-phase liquid chromatography. Utilizing a fast gradient, analysis time was under 11 minutes, but did not provide complete isomeric separation of 17ß-trenbolone and 17a-trenbolone, or 17ß-estradiol and 17a-estradiol. Column effluent underwent atmospheric-pressure chemical ionization (APCI) followed by detection using a triple-quadrupole mass spectrometer in SRM mode. The lower limit of quantitation (LLOQ) was =0.55 ng/filter for all compounds. Recoveries ranged from 93%-116%, and coefficient of variation (CV) was =16.1% at all spiking levels.

Original languageEnglish
Title of host publicationEvaluating Veterinary Pharmaceutical Behavior in the Environment
PublisherAmerican Chemical Society
Pages137-148
Number of pages12
ISBN (Print)9780841227019
DOIs
StatePublished - Mar 14 2013

Publication series

NameACS Symposium Series
Volume1126
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

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    Blackwell, B. R., Buser, M. D., Johnson, B. J., Baker, M., Cobb, G. P., & Smith, P. N. (2013). Analysis of veterinary growth promoters in airborne particulate matter by liquid chromatography-tandem mass spectrometry. In Evaluating Veterinary Pharmaceutical Behavior in the Environment (pp. 137-148). (ACS Symposium Series; Vol. 1126). American Chemical Society. https://doi.org/10.1021/bk-2013-1126.ch005