TY - GEN
T1 - Analysis of veterinary growth promoters in airborne particulate matter by liquid chromatography-tandem mass spectrometry
AU - Blackwell, Brett R.
AU - Buser, M. D.
AU - Johnson, B. J.
AU - Baker, M.
AU - Cobb, G. P.
AU - Smith, P. N.
PY - 2013/3/14
Y1 - 2013/3/14
N2 - Veterinary growth promoters are widely used throughout the United States in livestock production. In beef production, steroid hormones are administered to an estimated 90% of cattle. These include the synthetic steroids trenbolone acetate and melengestrol acetate, as well 17ß-estradiol. Given the endocrine-modulating activity of steroid growth promoters, a sensitive and reliable analytical method is needed to detect trenbolone, melengestrol acetate, estradiol and related residues in environmental matrices. We have developed a method that incorporates solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry for he simultaneous determination of trendione, trenbolone, melengestrol acetate, estrone, and estradiol in airborne particulate matter samples. Sample preparation involved liquid-solid extraction followed by cleanup with SPE cartridges. Analytes were separated using reversed-phase liquid chromatography. Utilizing a fast gradient, analysis time was under 11 minutes, but did not provide complete isomeric separation of 17ß-trenbolone and 17a-trenbolone, or 17ß-estradiol and 17a-estradiol. Column effluent underwent atmospheric-pressure chemical ionization (APCI) followed by detection using a triple-quadrupole mass spectrometer in SRM mode. The lower limit of quantitation (LLOQ) was =0.55 ng/filter for all compounds. Recoveries ranged from 93%-116%, and coefficient of variation (CV) was =16.1% at all spiking levels.
AB - Veterinary growth promoters are widely used throughout the United States in livestock production. In beef production, steroid hormones are administered to an estimated 90% of cattle. These include the synthetic steroids trenbolone acetate and melengestrol acetate, as well 17ß-estradiol. Given the endocrine-modulating activity of steroid growth promoters, a sensitive and reliable analytical method is needed to detect trenbolone, melengestrol acetate, estradiol and related residues in environmental matrices. We have developed a method that incorporates solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry for he simultaneous determination of trendione, trenbolone, melengestrol acetate, estrone, and estradiol in airborne particulate matter samples. Sample preparation involved liquid-solid extraction followed by cleanup with SPE cartridges. Analytes were separated using reversed-phase liquid chromatography. Utilizing a fast gradient, analysis time was under 11 minutes, but did not provide complete isomeric separation of 17ß-trenbolone and 17a-trenbolone, or 17ß-estradiol and 17a-estradiol. Column effluent underwent atmospheric-pressure chemical ionization (APCI) followed by detection using a triple-quadrupole mass spectrometer in SRM mode. The lower limit of quantitation (LLOQ) was =0.55 ng/filter for all compounds. Recoveries ranged from 93%-116%, and coefficient of variation (CV) was =16.1% at all spiking levels.
UR - http://www.scopus.com/inward/record.url?scp=84905364103&partnerID=8YFLogxK
U2 - 10.1021/bk-2013-1126.ch005
DO - 10.1021/bk-2013-1126.ch005
M3 - Conference contribution
AN - SCOPUS:84905364103
SN - 9780841227019
T3 - ACS Symposium Series
SP - 137
EP - 148
BT - Evaluating Veterinary Pharmaceutical Behavior in the Environment
PB - American Chemical Society
ER -