Analysis of permethylated glycan by liquid chromatography (LC) and mass spectrometry (MS)

Shiyue Zhou, Kerry M. Wooding, Yehia Mechref

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

13 Scopus citations

Abstract

The development of a reliable and high-throughput glycomic profiling strategy is in high demand due to the biological roles of glycans and their association with different diseases. Native analysis can be quite difficult because of the low ionization efficiency and microheterogeneity of glycans. In this chapter, the sample preparation protocols and LC-MS analysis of permethylated glycan strategies are introduced. Solid-phase permethylation is a fast, convenient, and high-yield method to stabilize sialic acid and improve gly-can ionization efficiency and analysis in positive mode; this results in a more sensitive and reliable glycomic profiling strategy. Several modifications in the LC method are also mentioned in this chapter. Online purification simplifies sample preparation and reduces sample loss. Elevating the column temperature significantly improves the peak shape of permethylated glycans and results in isomeric separation. The identification and quantification of permethylated glycans can be achieved through high resolution MS and MS/MS experiments using a MRM method; both approaches are reliable, sensitive, and conducive to high-throughput glycomic studies.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages83-96
Number of pages14
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1503
ISSN (Print)1064-3745

Keywords

  • LC-MS
  • MRM
  • Online purification
  • Permethylation
  • Quantitative glycomics

Fingerprint Dive into the research topics of 'Analysis of permethylated glycan by liquid chromatography (LC) and mass spectrometry (MS)'. Together they form a unique fingerprint.

Cite this