TY - JOUR
T1 - Analysis of intense, subnanosecond electrical pulse-induced transmembrane voltage in spheroidal cells with arbitrary orientation
AU - Hu, Qin
AU - Joshi, Ravindra P.
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009/6
Y1 - 2009/6
N2 - Self-consistent evaluations of the transmembrane potential (TMP) and possible membrane electroporation in spheroidal cells arising from an ultrashort, high-intensity pulse are reported. The present study couples the Laplace equation with Smoluchowski theory of pore formation, and uses double-shell models. It is shown that the response of prolate spheroids is faster than that of the sphere, with the outer membrane reaching its steady-state value in about 2 μs. The simulation result also shows that the TMP across an inner organelle could exceed the value across the plasma membrane at least over the first 0.4 μs or so, indicating a possibility of intracellular, electromanipulation of cells. The TMP induced by pulsed external voltages is predicted to be higher in oblate spheroids in comparison to both spherical and prolate spheroidal cells. This occurs due to flattening of the surface area.
AB - Self-consistent evaluations of the transmembrane potential (TMP) and possible membrane electroporation in spheroidal cells arising from an ultrashort, high-intensity pulse are reported. The present study couples the Laplace equation with Smoluchowski theory of pore formation, and uses double-shell models. It is shown that the response of prolate spheroids is faster than that of the sphere, with the outer membrane reaching its steady-state value in about 2 μs. The simulation result also shows that the TMP across an inner organelle could exceed the value across the plasma membrane at least over the first 0.4 μs or so, indicating a possibility of intracellular, electromanipulation of cells. The TMP induced by pulsed external voltages is predicted to be higher in oblate spheroids in comparison to both spherical and prolate spheroidal cells. This occurs due to flattening of the surface area.
KW - Bioelectric simulation
KW - Cellular electroporation
KW - Spheroidal cells
KW - Transmembrane voltage
KW - Ultrashort pulse
UR - http://www.scopus.com/inward/record.url?scp=67650626814&partnerID=8YFLogxK
U2 - 10.1109/TBME.2009.2015459
DO - 10.1109/TBME.2009.2015459
M3 - Article
C2 - 19258194
AN - SCOPUS:67650626814
VL - 56
SP - 1617
EP - 1626
JO - IEEE Transactions on Biomedical Engineering
JF - IEEE Transactions on Biomedical Engineering
SN - 0018-9294
IS - 6
M1 - 4796243
ER -