TY - JOUR
T1 - Analysis of intense, subnanosecond electrical pulse-induced transmembrane voltage in spheroidal cells with arbitrary orientation
AU - Hu, Qin
AU - Joshi, Ravindra P.
N1 - Funding Information:
Manuscript received September 1, 2008; revised December 1, 2008. First published February 27, 2009; current version published June 10, 2009. This work was supported in part by a grant from Central Michigan University. Asterisk indicates corresponding author. Q. Hu is with the Department of Engineering and Technology, Central Michigan University, Mt. Pleasant, MI 48859 USA (e-mail: hu1q@cmich.edu). ∗R. P. Joshi is with the Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529-0246 USA (e-mail: rjoshi@odu.edu). Color versions of one or more of the figures in this paper are available online at http://ieeexplore.ieee.org. Digital Object Identifier 10.1109/TBME.2009.2015459
PY - 2009/6
Y1 - 2009/6
N2 - Self-consistent evaluations of the transmembrane potential (TMP) and possible membrane electroporation in spheroidal cells arising from an ultrashort, high-intensity pulse are reported. The present study couples the Laplace equation with Smoluchowski theory of pore formation, and uses double-shell models. It is shown that the response of prolate spheroids is faster than that of the sphere, with the outer membrane reaching its steady-state value in about 2 μs. The simulation result also shows that the TMP across an inner organelle could exceed the value across the plasma membrane at least over the first 0.4 μs or so, indicating a possibility of intracellular, electromanipulation of cells. The TMP induced by pulsed external voltages is predicted to be higher in oblate spheroids in comparison to both spherical and prolate spheroidal cells. This occurs due to flattening of the surface area.
AB - Self-consistent evaluations of the transmembrane potential (TMP) and possible membrane electroporation in spheroidal cells arising from an ultrashort, high-intensity pulse are reported. The present study couples the Laplace equation with Smoluchowski theory of pore formation, and uses double-shell models. It is shown that the response of prolate spheroids is faster than that of the sphere, with the outer membrane reaching its steady-state value in about 2 μs. The simulation result also shows that the TMP across an inner organelle could exceed the value across the plasma membrane at least over the first 0.4 μs or so, indicating a possibility of intracellular, electromanipulation of cells. The TMP induced by pulsed external voltages is predicted to be higher in oblate spheroids in comparison to both spherical and prolate spheroidal cells. This occurs due to flattening of the surface area.
KW - Bioelectric simulation
KW - Cellular electroporation
KW - Spheroidal cells
KW - Transmembrane voltage
KW - Ultrashort pulse
UR - http://www.scopus.com/inward/record.url?scp=67650626814&partnerID=8YFLogxK
U2 - 10.1109/TBME.2009.2015459
DO - 10.1109/TBME.2009.2015459
M3 - Article
C2 - 19258194
AN - SCOPUS:67650626814
VL - 56
SP - 1617
EP - 1626
JO - IEEE Transactions on Biomedical Engineering
JF - IEEE Transactions on Biomedical Engineering
SN - 0018-9294
IS - 6
M1 - 4796243
ER -