TY - JOUR
T1 - Analysis of gene expression during adipogenesis in 3T3‐F442A preadipocytes
T2 - Insulin and dexamethasone control
AU - Moustaid, Naïma
AU - Lasnier, Françoise
AU - Hainque, Bernard
AU - Quignard‐Boulange, Annie
AU - Pairault, Jacques
PY - 1990/4
Y1 - 1990/4
N2 - In the present study, we have investigated dexamethasone and insulin regulation of the expression of adipose‐specific mRNA, namely, glycerophosphate dehydrogenase (G3PDH) and adipsin, at different stages of differentiation. During adipose conversion, insulin promotes an accumulation of G3PDH mRNA which is linked to cell differentiation; in fully differentiated cells, insulin is not required to maintain G3PDH gene expression. Differentiating cells in serum deprived medium already exhibit, at day I, a maximal amount of mRNA encoding for adipsin, which is tenfold decreased by 10 nM of insulin; insulin also exerts a negative effect on the abundance of adipsin mRNA in mature cells. This result indicates that adipsin appears to be a very early marker of adipose conversion, the gene expression of which is down‐regulated by the presence of insulin. Dexamethasone (DEX) decreases the G3PDH message at all stages of adipose conversion, while it promotes the accumulation of adipsin mRNA mainly in differentiating cells. In DEX‐treated adipocytes, the transcription efficiency of the G3PDH gene is not altered, and reduction to 50% of the message is due essentially to an approximately twofold decrease in its half‐life.
AB - In the present study, we have investigated dexamethasone and insulin regulation of the expression of adipose‐specific mRNA, namely, glycerophosphate dehydrogenase (G3PDH) and adipsin, at different stages of differentiation. During adipose conversion, insulin promotes an accumulation of G3PDH mRNA which is linked to cell differentiation; in fully differentiated cells, insulin is not required to maintain G3PDH gene expression. Differentiating cells in serum deprived medium already exhibit, at day I, a maximal amount of mRNA encoding for adipsin, which is tenfold decreased by 10 nM of insulin; insulin also exerts a negative effect on the abundance of adipsin mRNA in mature cells. This result indicates that adipsin appears to be a very early marker of adipose conversion, the gene expression of which is down‐regulated by the presence of insulin. Dexamethasone (DEX) decreases the G3PDH message at all stages of adipose conversion, while it promotes the accumulation of adipsin mRNA mainly in differentiating cells. In DEX‐treated adipocytes, the transcription efficiency of the G3PDH gene is not altered, and reduction to 50% of the message is due essentially to an approximately twofold decrease in its half‐life.
KW - adipsin mRNA's
KW - differentiation
KW - glucocorticoids
KW - glycerophosphate dehydrogenase
KW - insulin
UR - http://www.scopus.com/inward/record.url?scp=0025305822&partnerID=8YFLogxK
U2 - 10.1002/jcb.240420407
DO - 10.1002/jcb.240420407
M3 - Article
C2 - 2187890
AN - SCOPUS:0025305822
SN - 0730-2312
VL - 42
SP - 243
EP - 254
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
IS - 4
ER -