Analysis of complex O-linked glycans by capillary elecrtrochromatography/ fourier transform mass spectrometry

Yehia Mechref, Amy Que, Yunping Huang, John A. Taraszka, David E. Clemmer, Milos V. Novotny

Research output: Contribution to conferencePaperpeer-review

Abstract

The capillary electrochromatography (CEC)-ESI Fourier transform mass spectrometry (FT/MS) technique was developed for the identification and characterization of complex mixtures of oligosaccharides and O-glycans. CEC columns were made using gels consisting of 5% T, 60% C, 3% PEG, 40% 2-cyanoethyl acrylate and 10% vinylsulfonic acid. Columns were interfaced with the FT/MS using pulled fused silica needles of larger inner diameters. Results show that the high mass resolution of FT/MS enabled the resolution of coeluting components in a mixture.

Original languageEnglish
Pages71-72
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Conference

ConferenceProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL
Period06/2/0206/6/02

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