Analysis of complex O-linked glycans by capillary elecrtrochromatography/ fourier transform mass spectrometry

Yehia Mechref; Amy Que; Yunping Huang; John A. Taraszka; David E. Clemmer; Milos V. Novotny

Analysis of complex O-linked glycans by capillary elecrtrochromatography/ fourier transform mass spectrometry

Yehia Mechref, Amy Que, Yunping Huang, John A. Taraszka, David E. Clemmer, Milos V. Novotny

Chemistry and Biochemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

The capillary electrochromatography (CEC)-ESI Fourier transform mass spectrometry (FT/MS) technique was developed for the identification and characterization of complex mixtures of oligosaccharides and O-glycans. CEC columns were made using gels consisting of 5% T, 60% C, 3% PEG, 40% 2-cyanoethyl acrylate and 10% vinylsulfonic acid. Columns were interfaced with the FT/MS using pulled fused silica needles of larger inner diameters. Results show that the high mass resolution of FT/MS enabled the resolution of coeluting components in a mixture.

Original language	English
Pages	71-72
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	06/2/02 → 06/6/02

Cite this

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title = "Analysis of complex O-linked glycans by capillary elecrtrochromatography/ fourier transform mass spectrometry",

abstract = "The capillary electrochromatography (CEC)-ESI Fourier transform mass spectrometry (FT/MS) technique was developed for the identification and characterization of complex mixtures of oligosaccharides and O-glycans. CEC columns were made using gels consisting of 5% T, 60% C, 3% PEG, 40% 2-cyanoethyl acrylate and 10% vinylsulfonic acid. Columns were interfaced with the FT/MS using pulled fused silica needles of larger inner diameters. Results show that the high mass resolution of FT/MS enabled the resolution of coeluting components in a mixture.",

author = "Yehia Mechref and Amy Que and Yunping Huang and Taraszka, {John A.} and Clemmer, {David E.} and Novotny, {Milos V.}",

year = "2002",

language = "English",

pages = "71--72",

note = "null ; Conference date: 02-06-2002 Through 06-06-2002",

}

Analysis of complex O-linked glycans by capillary elecrtrochromatography/ fourier transform mass spectrometry. / Mechref, Yehia; Que, Amy; Huang, Yunping; Taraszka, John A.; Clemmer, David E.; Novotny, Milos V.

2002. 71-72 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Analysis of complex O-linked glycans by capillary elecrtrochromatography/ fourier transform mass spectrometry

AU - Mechref, Yehia

AU - Que, Amy

AU - Huang, Yunping

AU - Taraszka, John A.

AU - Clemmer, David E.

AU - Novotny, Milos V.

PY - 2002

Y1 - 2002

N2 - The capillary electrochromatography (CEC)-ESI Fourier transform mass spectrometry (FT/MS) technique was developed for the identification and characterization of complex mixtures of oligosaccharides and O-glycans. CEC columns were made using gels consisting of 5% T, 60% C, 3% PEG, 40% 2-cyanoethyl acrylate and 10% vinylsulfonic acid. Columns were interfaced with the FT/MS using pulled fused silica needles of larger inner diameters. Results show that the high mass resolution of FT/MS enabled the resolution of coeluting components in a mixture.

AB - The capillary electrochromatography (CEC)-ESI Fourier transform mass spectrometry (FT/MS) technique was developed for the identification and characterization of complex mixtures of oligosaccharides and O-glycans. CEC columns were made using gels consisting of 5% T, 60% C, 3% PEG, 40% 2-cyanoethyl acrylate and 10% vinylsulfonic acid. Columns were interfaced with the FT/MS using pulled fused silica needles of larger inner diameters. Results show that the high mass resolution of FT/MS enabled the resolution of coeluting components in a mixture.

UR - http://www.scopus.com/inward/record.url?scp=2442671661&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442671661

SP - 71

EP - 72

Y2 - 2 June 2002 through 6 June 2002

ER -

Analysis of complex O-linked glycans by capillary elecrtrochromatography/ fourier transform mass spectrometry

Abstract

Conference

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