TY - JOUR
T1 - Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems
AU - Rossi, M.
AU - Carrari, F.
AU - Cabrera-Ponce, J. L.
AU - Vázquez-Rovere, C.
AU - Herrera-Estrella, L.
AU - Gudesblat, G.
AU - Iusem, N. D.
N1 - Funding Information:
Acknowledgments The authors are grateful to Dr. Alejandro Mentaberry for providing growth chamber facilities. We thank Dr. Armando Parodi for revising the manuscript and Dr. Andrew Thompson for useful discussion and for overall advice. CINVES-TAV-IPN thanks Guadalupe Sánchez-García for her excellent assistance in tissue culture and transformation of papaya and Antonio Cisneros for the photographic work. M. R. holds a fellowship from the University of Buenos Aires. F. C. is indebted to UNESCO for a training fellowship. This work was funded by CINVESTAV, Mexico and partly by grants from the University of Buenos Aires and Fundación Antorchas, Argentina.
PY - 1998
Y1 - 1998
N2 - Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To prove that the promoter region is functional in vivo, we fused it upstream of the β-glucuronidase (GUS) reporter gene. The resulting chimeric gene fusion was used for transient expression assays in papaya embryogenic calli and leaves. In addition, the same construct was used to produce transgenic tomato, papaya, tobacco, and potato plants. Asr2 upstream sequences showed promoter function in all of these systems. Under the experimental conditions tested, ABA stimulated GUS expression in papaya and tobacco, but not in tomato and potato systems.
AB - Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To prove that the promoter region is functional in vivo, we fused it upstream of the β-glucuronidase (GUS) reporter gene. The resulting chimeric gene fusion was used for transient expression assays in papaya embryogenic calli and leaves. In addition, the same construct was used to produce transgenic tomato, papaya, tobacco, and potato plants. Asr2 upstream sequences showed promoter function in all of these systems. Under the experimental conditions tested, ABA stimulated GUS expression in papaya and tobacco, but not in tomato and potato systems.
KW - Abscisic acid (ABA)
KW - Asr genes
KW - Asr2
KW - Drought stress
KW - Tomato
UR - http://www.scopus.com/inward/record.url?scp=0031900283&partnerID=8YFLogxK
U2 - 10.1007/s004380050700
DO - 10.1007/s004380050700
M3 - Article
C2 - 9613566
AN - SCOPUS:0031900283
SN - 0026-8925
VL - 258
SP - 1
EP - 8
JO - Molecular and General Genetics
JF - Molecular and General Genetics
IS - 1-2
ER -