TY - JOUR
T1 - An update on smart biocatalysts for industrial and biomedical applications
AU - Shakya, Akhilesh Kumar
AU - Nandakumar, Kutty Selva
N1 - Funding Information:
Currently, the research of K.S.N. is supported by a grant (C1034211) from Southern Medical University, Guangzhou, People's Republic of China.
Publisher Copyright:
© 2018 The Author(s) Published by the Royal Society. All rights reserved.
PY - 2018
Y1 - 2018
N2 - Recently, smart biocatalysts, where enzymes are conjugated to stimuli-responsive (smart) polymers, have gained significant attention. Based on the presence or absence of external stimuli, the polymer attached to the enzyme changes its conformation to protect the enzyme from the external environment and regulate the enzyme activity, thus acting as a molecular switch. Owing to this behaviour, smart biocatalysts can be separated easily from a reaction mixture and re-used several times. Several such smart polymer-based biocatalysts have been developed for industrial and biomedical applications. In addition, they have been used in biosensors, biometrics and nano-electronic devices. This review article covers recent advances in developing different kinds of stimuli-responsive enzyme bioconjugates, including conjugation strategies, and their applications.
AB - Recently, smart biocatalysts, where enzymes are conjugated to stimuli-responsive (smart) polymers, have gained significant attention. Based on the presence or absence of external stimuli, the polymer attached to the enzyme changes its conformation to protect the enzyme from the external environment and regulate the enzyme activity, thus acting as a molecular switch. Owing to this behaviour, smart biocatalysts can be separated easily from a reaction mixture and re-used several times. Several such smart polymer-based biocatalysts have been developed for industrial and biomedical applications. In addition, they have been used in biosensors, biometrics and nano-electronic devices. This review article covers recent advances in developing different kinds of stimuli-responsive enzyme bioconjugates, including conjugation strategies, and their applications.
KW - Industrial biocatalyst
KW - Magnetic smart biocatalyst
KW - Responsive polymer
KW - Smart biocatalyst
KW - Therapeutic biocatalyst
KW - Thermo-responsive biocatalyst
UR - http://www.scopus.com/inward/record.url?scp=85048874949&partnerID=8YFLogxK
U2 - 10.1098/rsif.2018.0062
DO - 10.1098/rsif.2018.0062
M3 - Review article
C2 - 29491182
AN - SCOPUS:85048874949
SN - 1742-5689
VL - 15
JO - Journal of the Royal Society Interface
JF - Journal of the Royal Society Interface
IS - 139
M1 - 20180062
ER -