TY - JOUR
T1 - An enzyme-release assay for natural cytotoxicity
AU - Korzeniewski, Carol
AU - Callewaert, Denis M.
N1 - Funding Information:
i This investigation was supported by PHS grants AI-12766 and CA-34112 from the National Institutes of Health. 2 Recipient of Research Career Development Award CA-00744 from the National Cancer Institute. To whom all correspondence should be addressed.
PY - 1983/11/25
Y1 - 1983/11/25
N2 - A sensitive enzyme-release assay for natural cytotoxicity is described. Thekinetic determination of the amount of the enzyme lactate dehydrogenase (LDH) released from lysed target cells was determined to provide a sensitive and precise measure of natural cytotoxicity when used in conjunction with appropriate controls and calculational methods. Values for the percentage of cytotoxicity or kinetic parameters determined by this method were identical, within experimental error, to values determined in parallel 51Cr release assays. Moreover, it was found that the spontaneous release of LDH from the target cells tested was considerably lower than the spontaneous release of 51Cr. This enzyme-release cytoxocity assay is convenient, inexpensive, and precise, and should be applicable to the study of other cytotoxicity reactions, including antibody-dependent and T-cell mediated reactions.
AB - A sensitive enzyme-release assay for natural cytotoxicity is described. Thekinetic determination of the amount of the enzyme lactate dehydrogenase (LDH) released from lysed target cells was determined to provide a sensitive and precise measure of natural cytotoxicity when used in conjunction with appropriate controls and calculational methods. Values for the percentage of cytotoxicity or kinetic parameters determined by this method were identical, within experimental error, to values determined in parallel 51Cr release assays. Moreover, it was found that the spontaneous release of LDH from the target cells tested was considerably lower than the spontaneous release of 51Cr. This enzyme-release cytoxocity assay is convenient, inexpensive, and precise, and should be applicable to the study of other cytotoxicity reactions, including antibody-dependent and T-cell mediated reactions.
KW - cell mediated cytotoxicity
KW - enzyme-release assay
KW - lactate dehydrogenase
KW - natural cytotoxicity
KW - quantification of cytotoxicity reactions
UR - http://www.scopus.com/inward/record.url?scp=0021038289&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(83)90438-6
DO - 10.1016/0022-1759(83)90438-6
M3 - Article
C2 - 6199426
AN - SCOPUS:0021038289
VL - 64
SP - 313
EP - 320
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 3
ER -