An enzyme-release assay for natural cytotoxicity

Carol Korzeniewski; Denis M. Callewaert

doi:10.1016/0022-1759(83)90438-6

An enzyme-release assay for natural cytotoxicity

Carol Korzeniewski, Denis M. Callewaert

Chemistry and Biochemistry

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869 Scopus citations

Abstract

A sensitive enzyme-release assay for natural cytotoxicity is described. Thekinetic determination of the amount of the enzyme lactate dehydrogenase (LDH) released from lysed target cells was determined to provide a sensitive and precise measure of natural cytotoxicity when used in conjunction with appropriate controls and calculational methods. Values for the percentage of cytotoxicity or kinetic parameters determined by this method were identical, within experimental error, to values determined in parallel ⁵¹Cr release assays. Moreover, it was found that the spontaneous release of LDH from the target cells tested was considerably lower than the spontaneous release of ⁵¹Cr. This enzyme-release cytoxocity assay is convenient, inexpensive, and precise, and should be applicable to the study of other cytotoxicity reactions, including antibody-dependent and T-cell mediated reactions.

Original language	English
Pages (from-to)	313-320
Number of pages	8
Journal	Journal of Immunological Methods
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/0022-1759(83)90438-6
State	Published - Nov 25 1983

Keywords

cell mediated cytotoxicity
enzyme-release assay
lactate dehydrogenase
natural cytotoxicity
quantification of cytotoxicity reactions

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10.1016/0022-1759(83)90438-6

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title = "An enzyme-release assay for natural cytotoxicity",

abstract = "A sensitive enzyme-release assay for natural cytotoxicity is described. Thekinetic determination of the amount of the enzyme lactate dehydrogenase (LDH) released from lysed target cells was determined to provide a sensitive and precise measure of natural cytotoxicity when used in conjunction with appropriate controls and calculational methods. Values for the percentage of cytotoxicity or kinetic parameters determined by this method were identical, within experimental error, to values determined in parallel 51Cr release assays. Moreover, it was found that the spontaneous release of LDH from the target cells tested was considerably lower than the spontaneous release of 51Cr. This enzyme-release cytoxocity assay is convenient, inexpensive, and precise, and should be applicable to the study of other cytotoxicity reactions, including antibody-dependent and T-cell mediated reactions.",

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AU - Korzeniewski, Carol

AU - Callewaert, Denis M.

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N2 - A sensitive enzyme-release assay for natural cytotoxicity is described. Thekinetic determination of the amount of the enzyme lactate dehydrogenase (LDH) released from lysed target cells was determined to provide a sensitive and precise measure of natural cytotoxicity when used in conjunction with appropriate controls and calculational methods. Values for the percentage of cytotoxicity or kinetic parameters determined by this method were identical, within experimental error, to values determined in parallel 51Cr release assays. Moreover, it was found that the spontaneous release of LDH from the target cells tested was considerably lower than the spontaneous release of 51Cr. This enzyme-release cytoxocity assay is convenient, inexpensive, and precise, and should be applicable to the study of other cytotoxicity reactions, including antibody-dependent and T-cell mediated reactions.

AB - A sensitive enzyme-release assay for natural cytotoxicity is described. Thekinetic determination of the amount of the enzyme lactate dehydrogenase (LDH) released from lysed target cells was determined to provide a sensitive and precise measure of natural cytotoxicity when used in conjunction with appropriate controls and calculational methods. Values for the percentage of cytotoxicity or kinetic parameters determined by this method were identical, within experimental error, to values determined in parallel 51Cr release assays. Moreover, it was found that the spontaneous release of LDH from the target cells tested was considerably lower than the spontaneous release of 51Cr. This enzyme-release cytoxocity assay is convenient, inexpensive, and precise, and should be applicable to the study of other cytotoxicity reactions, including antibody-dependent and T-cell mediated reactions.

KW - cell mediated cytotoxicity

KW - enzyme-release assay

KW - lactate dehydrogenase

KW - natural cytotoxicity

KW - quantification of cytotoxicity reactions

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