An Empirical Bayesian Approach for Identifying Differential Coexpression in High-Throughput Experiments

John A. Dawson, Christina Kendziorski

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

A common goal of microarray and related high-throughput genomic experiments is to identify genes that vary across biological condition. Most often this is accomplished by identifying genes with changes in mean expression level, so called differentially expressed (DE) genes, and a number of effective methods for identifying DE genes have been developed. Although useful, these approaches do not accommodate other types of differential regulation. An important example concerns differential coexpression (DC). Investigations of this class of genes are hampered by the large cardinality of the space to be interrogated as well as by influential outliers. As a result, existing DC approaches are often underpowered, exceedingly prone to false discoveries, and/or computationally intractable for even a moderately large number of pairs. To address this, an empirical Bayesian approach for identifying DC gene pairs is developed. The approach provides a false discovery rate controlled list of significant DC gene pairs without sacrificing power. It is applicable within a single study as well as across multiple studies. Computations are greatly facilitated by a modification to the expectation-maximization algorithm and a procedural heuristic. Simulations suggest that the proposed approach outperforms existing methods in far less computational time; and case study results suggest that the approach will likely prove to be a useful complement to current DE methods in high-throughput genomic studies.

Original languageEnglish
Pages (from-to)455-465
Number of pages11
JournalBiometrics
Volume68
Issue number2
DOIs
StatePublished - Jun 2012

Keywords

  • Coexpression
  • Differential expression
  • Empirical bayes
  • Gene expression
  • Meta-analysis
  • Microarray

Fingerprint Dive into the research topics of 'An Empirical Bayesian Approach for Identifying Differential Coexpression in High-Throughput Experiments'. Together they form a unique fingerprint.

  • Cite this