An electrically active microneedle array for electroporation of skin for gene delivery

Seong O. Choi; Jung Hwan Park; Yoonsu Choi; Yeuchun Kim; Harvinder S. Gill; Yong Kyu Yoon; Mark R. Prausnitz; Mark G. Allen

An electrically active microneedle array for electroporation of skin for gene delivery

Seong O. Choi, Jung Hwan Park, Yoonsu Choi, Yeuchun Kim, Harvinder S. Gill, Yong Kyu Yoon, Mark R. Prausnitz, Mark G. Allen

Chemical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Scopus citations

Abstract

We have designed and fabricated a microneedle array with electrical functionality with the final goal of electroporating skin's epidermal cells to increase their transfection by DNA vaccines. The microneedle array was made of polymethylmethacrylate (PMMA) by micromolding technology from a master PDMS mold, followed by metal deposition, patterning using laser ablation, and electrodeposition. This microneedle array possessed sufficient mechanical strength to penetrate human skin in vivo and was also able to electroporate red blood cells as an in vitro model to demonstrate cell membrane permeabilization.

Original language	English
Title of host publication	TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers
Pages	1513-1516
Number of pages	4
State	Published - 2005
Event	13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05 - Seoul, Korea, Republic of Duration: Jun 5 2005 → Jun 9 2005

Publication series

Name	Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05
Volume	2

Conference

Conference	13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05
Country	Korea, Republic of
City	Seoul
Period	06/5/05 → 06/9/05

Keywords

Electroporation
Gene delivery
Laser ablation
Micromolding
Microneedle

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Choi, S. O., Park, J. H., Choi, Y., Kim, Y., Gill, H. S., Yoon, Y. K., Prausnitz, M. R., & Allen, M. G. (2005). An electrically active microneedle array for electroporation of skin for gene delivery. In TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers (pp. 1513-1516). [3E4.54] (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05; Vol. 2).

Choi, Seong O. ; Park, Jung Hwan ; Choi, Yoonsu ; Kim, Yeuchun ; Gill, Harvinder S. ; Yoon, Yong Kyu ; Prausnitz, Mark R. ; Allen, Mark G. / An electrically active microneedle array for electroporation of skin for gene delivery. TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. 2005. pp. 1513-1516 (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05).

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title = "An electrically active microneedle array for electroporation of skin for gene delivery",

abstract = "We have designed and fabricated a microneedle array with electrical functionality with the final goal of electroporating skin's epidermal cells to increase their transfection by DNA vaccines. The microneedle array was made of polymethylmethacrylate (PMMA) by micromolding technology from a master PDMS mold, followed by metal deposition, patterning using laser ablation, and electrodeposition. This microneedle array possessed sufficient mechanical strength to penetrate human skin in vivo and was also able to electroporate red blood cells as an in vitro model to demonstrate cell membrane permeabilization.",

keywords = "Electroporation, Gene delivery, Laser ablation, Micromolding, Microneedle",

author = "Choi, {Seong O.} and Park, {Jung Hwan} and Yoonsu Choi and Yeuchun Kim and Gill, {Harvinder S.} and Yoon, {Yong Kyu} and Prausnitz, {Mark R.} and Allen, {Mark G.}",

year = "2005",

language = "English",

isbn = "0780389948",

series = "Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05",

pages = "1513--1516",

booktitle = "TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers",

}

Choi, SO, Park, JH, Choi, Y, Kim, Y, Gill, HS, Yoon, YK, Prausnitz, MR & Allen, MG 2005, An electrically active microneedle array for electroporation of skin for gene delivery. in TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers., 3E4.54, Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05, vol. 2, pp. 1513-1516, 13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05, Seoul, Korea, Republic of, 06/5/05.

An electrically active microneedle array for electroporation of skin for gene delivery. / Choi, Seong O.; Park, Jung Hwan; Choi, Yoonsu; Kim, Yeuchun; Gill, Harvinder S.; Yoon, Yong Kyu; Prausnitz, Mark R.; Allen, Mark G.

TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. 2005. p. 1513-1516 3E4.54 (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - An electrically active microneedle array for electroporation of skin for gene delivery

AU - Choi, Seong O.

AU - Park, Jung Hwan

AU - Choi, Yoonsu

AU - Kim, Yeuchun

AU - Gill, Harvinder S.

AU - Yoon, Yong Kyu

AU - Prausnitz, Mark R.

AU - Allen, Mark G.

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N2 - We have designed and fabricated a microneedle array with electrical functionality with the final goal of electroporating skin's epidermal cells to increase their transfection by DNA vaccines. The microneedle array was made of polymethylmethacrylate (PMMA) by micromolding technology from a master PDMS mold, followed by metal deposition, patterning using laser ablation, and electrodeposition. This microneedle array possessed sufficient mechanical strength to penetrate human skin in vivo and was also able to electroporate red blood cells as an in vitro model to demonstrate cell membrane permeabilization.

AB - We have designed and fabricated a microneedle array with electrical functionality with the final goal of electroporating skin's epidermal cells to increase their transfection by DNA vaccines. The microneedle array was made of polymethylmethacrylate (PMMA) by micromolding technology from a master PDMS mold, followed by metal deposition, patterning using laser ablation, and electrodeposition. This microneedle array possessed sufficient mechanical strength to penetrate human skin in vivo and was also able to electroporate red blood cells as an in vitro model to demonstrate cell membrane permeabilization.

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KW - Gene delivery

KW - Laser ablation

KW - Micromolding

KW - Microneedle

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Choi SO, Park JH, Choi Y, Kim Y, Gill HS, Yoon YK et al. An electrically active microneedle array for electroporation of skin for gene delivery. In TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. 2005. p. 1513-1516. 3E4.54. (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05).