An electrically active microneedle array for electroporation

S. O. Choi, Y. C. Kim, J. H. Park, J. Hutcheson, Harvinder Gill, Y. K. Yoon, M. R. Prausnitz, M. G. Allen

Research output: Contribution to journalArticle

Abstract

We have designed and fabricated a microneedle array with electrical functionality with the final goal of electroporating skin’s epidermal cells to increase their transfection by DNA vaccines. The microneedle array was made of polymethylmethacrylate (PMMA) by micromolding technology from a polydimethylsiloxane (PDMS) mold, followed by metal deposition, patterning using laser ablation, and electrodeposition. This microneedle array possessed sufficient mechanical strength to penetrate human skin in vivo and was also able to electroporate both red blood cells and human prostate cancer cells as an in vitro model to demonstrate cell membrane permeabilization. A computational model to predict the effective volume for electroporation with respect to applied voltages was constructed from finite element simulation. This study demonstrates the mechanical and electrical functionalities of the first MEMS-fabricated microneedle array for electroporation, designed for DNA vaccine delivery.
Original languageEnglish
Pages (from-to)263-73
JournalBiomed Microdevices
StatePublished - 2010

Fingerprint Dive into the research topics of 'An electrically active microneedle array for electroporation'. Together they form a unique fingerprint.

  • Cite this

    Choi, S. O., Kim, Y. C., Park, J. H., Hutcheson, J., Gill, H., Yoon, Y. K., Prausnitz, M. R., & Allen, M. G. (2010). An electrically active microneedle array for electroporation. Biomed Microdevices, 263-73.