TY - JOUR
T1 - An efficient particle bombardment system for the genetic transformation of asparagus (Asparagus officinalis L.)
AU - Cabrera-Ponce, Jose Luis
AU - López, Liliana
AU - Assad-Garcia, Nacyra
AU - Medina-Arevalo, Consuelo
AU - Bailey, Ana Maria
AU - Herrera-Estrella, Luis
N1 - Funding Information:
We thank Azucena Mendoza, Mireya Sanchez, June Simpson and Gilberto Mozqueda for ideas and facilities for Southern blot analysis; Rosa Maria Rangel and Luis Cuahutemoc Navarro for helpful discussions about somatic embryogenesis, and Antonio Cisneros and Martha Martinez for the photographic work. This project was supported in part by a grant from the Irapuato asparagus growers association.
PY - 1997/2
Y1 - 1997/2
N2 - The microprojectile bombardment method was used to transfer DNA into embryogenic callus of asparagus (Asparagus officcinalis L.) and to produce stably transformed asparagus plants. Embryogenic callus, derived from UC157 and UC72 asparagus cultivars, was bombarded with tungsten particles coated with plasmid DNA that contained genes encoding hygromycin phosphotransferase, phosphinothricin acetyl transferase and β-glucuronidase. Putatively transformed calli were identified from the bombarded tissue after 4 months selection on 25 mg/L hygromycin B plus 4 mg/L phosphinothricin (PPT). By selecting embryogenic callus on hygromycin plus PPT the overall transformation and selection efficiencies were substantially improved over selection with hygromycin or PPT alone, where no transgenic clones were recovered. The transgenic nature of the selected material was demonstrated by GUS histochemical assays and Southern blot hybridization analysis. Transgenic asparagus plants were found to withstand the prescribed levels of the PPT-based herbicide BASTA(TM) for weed control.
AB - The microprojectile bombardment method was used to transfer DNA into embryogenic callus of asparagus (Asparagus officcinalis L.) and to produce stably transformed asparagus plants. Embryogenic callus, derived from UC157 and UC72 asparagus cultivars, was bombarded with tungsten particles coated with plasmid DNA that contained genes encoding hygromycin phosphotransferase, phosphinothricin acetyl transferase and β-glucuronidase. Putatively transformed calli were identified from the bombarded tissue after 4 months selection on 25 mg/L hygromycin B plus 4 mg/L phosphinothricin (PPT). By selecting embryogenic callus on hygromycin plus PPT the overall transformation and selection efficiencies were substantially improved over selection with hygromycin or PPT alone, where no transgenic clones were recovered. The transgenic nature of the selected material was demonstrated by GUS histochemical assays and Southern blot hybridization analysis. Transgenic asparagus plants were found to withstand the prescribed levels of the PPT-based herbicide BASTA(TM) for weed control.
UR - http://www.scopus.com/inward/record.url?scp=1842338052&partnerID=8YFLogxK
U2 - 10.1007/s002990050218
DO - 10.1007/s002990050218
M3 - Article
AN - SCOPUS:1842338052
VL - 16
SP - 255
EP - 260
JO - Plant Cell Reports
JF - Plant Cell Reports
SN - 0721-7714
IS - 5
ER -