TY - JOUR
T1 - An arabidopsis gene encoding a putative 14-3-3-interacting protein, caffeic acid/5-hydroxyferulic acid o-methyltransferase
AU - Zhang, Hong
AU - Wang, Jing
AU - Goodman, Howard M.
N1 - Funding Information:
We thank Dr. Randy Allen for his generous support in allowing us to use his laboratory to conduct some experiments. We thank Dr. Candance Haigler for critically reading the manuscript. This research is supported by a grant from Hoechst AG (Frankfurt, Germany) to H.M.G., and by the research enhancement award from Texas Tech University to H.Z.
PY - 1997/9/12
Y1 - 1997/9/12
N2 - AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. A caffeic acid/5-hydroxyferulic acid O-methyltransferase, OMT1, was identified as one of the several proteins that specifically interacts with AFT1 in yeast cells. The physical interaction between AFT1 and a partial OMT1 polypeptide can be demonstrated in vitro by using bacterially expressed proteins. A single copy gene was found to encode OMT1 in Arabidopsis, and its expression is both spatially and temporally regulated.
AB - AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. A caffeic acid/5-hydroxyferulic acid O-methyltransferase, OMT1, was identified as one of the several proteins that specifically interacts with AFT1 in yeast cells. The physical interaction between AFT1 and a partial OMT1 polypeptide can be demonstrated in vitro by using bacterially expressed proteins. A single copy gene was found to encode OMT1 in Arabidopsis, and its expression is both spatially and temporally regulated.
KW - 14-3-3 Protein
KW - Arabidopsis thaliana
KW - Caffeic acid
KW - Lignin biosynthesis
KW - O- Methyltransferase
KW - Tissue-specific expression
UR - http://www.scopus.com/inward/record.url?scp=0030869476&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(97)00096-1
DO - 10.1016/S0167-4781(97)00096-1
M3 - Article
C2 - 9349713
AN - SCOPUS:0030869476
SN - 0167-4781
VL - 1353
SP - 199
EP - 202
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 3
ER -