An arabidopsis gene encoding a putative 14-3-3-interacting protein, caffeic acid/5-hydroxyferulic acid o-methyltransferase

Hong Zhang, Jing Wang, Howard M. Goodman

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. A caffeic acid/5-hydroxyferulic acid O-methyltransferase, OMT1, was identified as one of the several proteins that specifically interacts with AFT1 in yeast cells. The physical interaction between AFT1 and a partial OMT1 polypeptide can be demonstrated in vitro by using bacterially expressed proteins. A single copy gene was found to encode OMT1 in Arabidopsis, and its expression is both spatially and temporally regulated.

Original languageEnglish
Pages (from-to)199-202
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1353
Issue number3
DOIs
StatePublished - Sep 12 1997

Keywords

  • 14-3-3 Protein
  • Arabidopsis thaliana
  • Caffeic acid
  • Lignin biosynthesis
  • O- Methyltransferase
  • Tissue-specific expression

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