An arabidopsis gene encoding a putative 14-3-3-interacting protein, caffeic acid/5-hydroxyferulic acid o-methyltransferase

Hong Zhang; Jing Wang; Howard M. Goodman

doi:10.1016/S0167-4781(97)00096-1

An arabidopsis gene encoding a putative 14-3-3-interacting protein, caffeic acid/5-hydroxyferulic acid o-methyltransferase

Hong Zhang, Jing Wang, Howard M. Goodman

Biological Sciences

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. A caffeic acid/5-hydroxyferulic acid O-methyltransferase, OMT1, was identified as one of the several proteins that specifically interacts with AFT1 in yeast cells. The physical interaction between AFT1 and a partial OMT1 polypeptide can be demonstrated in vitro by using bacterially expressed proteins. A single copy gene was found to encode OMT1 in Arabidopsis, and its expression is both spatially and temporally regulated.

Original language	English
Pages (from-to)	199-202
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1353
Issue number	3
DOIs	https://doi.org/10.1016/S0167-4781(97)00096-1
State	Published - Sep 12 1997

Keywords

14-3-3 Protein
Arabidopsis thaliana
Caffeic acid
Lignin biosynthesis
O- Methyltransferase
Tissue-specific expression

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10.1016/S0167-4781(97)00096-1

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title = "An arabidopsis gene encoding a putative 14-3-3-interacting protein, caffeic acid/5-hydroxyferulic acid o-methyltransferase",

abstract = "AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. A caffeic acid/5-hydroxyferulic acid O-methyltransferase, OMT1, was identified as one of the several proteins that specifically interacts with AFT1 in yeast cells. The physical interaction between AFT1 and a partial OMT1 polypeptide can be demonstrated in vitro by using bacterially expressed proteins. A single copy gene was found to encode OMT1 in Arabidopsis, and its expression is both spatially and temporally regulated.",

keywords = "14-3-3 Protein, Arabidopsis thaliana, Caffeic acid, Lignin biosynthesis, O- Methyltransferase, Tissue-specific expression",

author = "Hong Zhang and Jing Wang and Goodman, {Howard M.}",

note = "Funding Information: We thank Dr. Randy Allen for his generous support in allowing us to use his laboratory to conduct some experiments. We thank Dr. Candance Haigler for critically reading the manuscript. This research is supported by a grant from Hoechst AG (Frankfurt, Germany) to H.M.G., and by the research enhancement award from Texas Tech University to H.Z.",

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doi = "10.1016/S0167-4781(97)00096-1",

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AU - Zhang, Hong

AU - Wang, Jing

AU - Goodman, Howard M.

N1 - Funding Information: We thank Dr. Randy Allen for his generous support in allowing us to use his laboratory to conduct some experiments. We thank Dr. Candance Haigler for critically reading the manuscript. This research is supported by a grant from Hoechst AG (Frankfurt, Germany) to H.M.G., and by the research enhancement award from Texas Tech University to H.Z.

PY - 1997/9/12

Y1 - 1997/9/12

N2 - AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. A caffeic acid/5-hydroxyferulic acid O-methyltransferase, OMT1, was identified as one of the several proteins that specifically interacts with AFT1 in yeast cells. The physical interaction between AFT1 and a partial OMT1 polypeptide can be demonstrated in vitro by using bacterially expressed proteins. A single copy gene was found to encode OMT1 in Arabidopsis, and its expression is both spatially and temporally regulated.

AB - AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. A caffeic acid/5-hydroxyferulic acid O-methyltransferase, OMT1, was identified as one of the several proteins that specifically interacts with AFT1 in yeast cells. The physical interaction between AFT1 and a partial OMT1 polypeptide can be demonstrated in vitro by using bacterially expressed proteins. A single copy gene was found to encode OMT1 in Arabidopsis, and its expression is both spatially and temporally regulated.

KW - 14-3-3 Protein

KW - Arabidopsis thaliana

KW - Caffeic acid

KW - Lignin biosynthesis

KW - O- Methyltransferase

KW - Tissue-specific expression

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U2 - 10.1016/S0167-4781(97)00096-1

DO - 10.1016/S0167-4781(97)00096-1

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AN - SCOPUS:0030869476

VL - 1353

SP - 199

EP - 202

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 3

ER -

An arabidopsis gene encoding a putative 14-3-3-interacting protein, caffeic acid/5-hydroxyferulic acid o-methyltransferase

Abstract

Keywords

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