TY - JOUR
T1 - An arabidopsis gene encoding a putative 14-3-3-interacting protein, caffeic acid/5-hydroxyferulic acid o-methyltransferase
AU - Zhang, Hong
AU - Wang, Jing
AU - Goodman, Howard M.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1997/9/12
Y1 - 1997/9/12
N2 - AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. A caffeic acid/5-hydroxyferulic acid O-methyltransferase, OMT1, was identified as one of the several proteins that specifically interacts with AFT1 in yeast cells. The physical interaction between AFT1 and a partial OMT1 polypeptide can be demonstrated in vitro by using bacterially expressed proteins. A single copy gene was found to encode OMT1 in Arabidopsis, and its expression is both spatially and temporally regulated.
AB - AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. A caffeic acid/5-hydroxyferulic acid O-methyltransferase, OMT1, was identified as one of the several proteins that specifically interacts with AFT1 in yeast cells. The physical interaction between AFT1 and a partial OMT1 polypeptide can be demonstrated in vitro by using bacterially expressed proteins. A single copy gene was found to encode OMT1 in Arabidopsis, and its expression is both spatially and temporally regulated.
KW - 14-3-3 Protein
KW - Arabidopsis thaliana
KW - Caffeic acid
KW - Lignin biosynthesis
KW - O- Methyltransferase
KW - Tissue-specific expression
UR - http://www.scopus.com/inward/record.url?scp=0030869476&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(97)00096-1
DO - 10.1016/S0167-4781(97)00096-1
M3 - Article
C2 - 9349713
AN - SCOPUS:0030869476
VL - 1353
SP - 199
EP - 202
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 3
ER -