An alternative pathway for plant in vitro regeneration of insecticidal tree Melia azedarach L. derived from the induction of somatic embryogenesis

S. Sharry, W. Abedini, S. Lede, J. L. Cabrera Ponce, L. Herrera Estrella, R. M. Rangel Cano

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

A model for highly efficient method for plant regeneration from somatic embryos of chinaberry tree (Melia azedarach L.) was obtained. Mature seeds were cultured on Murashige and Skoog's medium supplemented with NAA/BA to induce embryogenic tissues. Embryo proliferation occurred with high level citokinin concentration (10 mg/L BAP) and germination occurred on free hormone medium or in BAP/AG3 medium. Plants were regenerated from both, direct embryo formation and through an intermediary callus phase. Results showed 100% embryo conversion to plant. Regenerated plants were normal phenotypically and continued to grow after transfer to a greenhouse environment with 100% of surviving with high rate of growth.

Original languageEnglish
Title of host publicationProceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding
PublisherInternational Society for Horticultural Science
Pages489-494
Number of pages6
ISBN (Print)906605719X, 9789066057197
DOIs
StatePublished - Nov 2006

Publication series

NameActa Horticulturae
Volume725 I
ISSN (Print)0567-7572

Keywords

  • Adenine
  • Direct and indirect morphogenesis
  • Melia azedarach
  • Plant regeneration
  • Somatic embryogenesis

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