An algorithm for neurite outgrowth reconstruction

Christina M. Weaver, John D. Pinezich, W. Brent Lindquist, Marcelo E. Vazquez

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


We present a numerical method which provides the ability to analyze digitized microscope images of retinal explants and quantify neurite outgrowth. Few parameters are required as input and limited user interaction is necessary to process an entire experiment of images. This eliminates fatigue related errors and user-related bias common to manual analysis. The method does not rely on stained images and handles images of variable quality. The algorithm is used to determine time and dose dependent, in vitro, neurotoxic effects of 1 GeV per nucleon iron particles in retinal explants. No neurotoxic effects are detected until 72 h after exposure; at 72 h, significant reductions of neurite outgrowth occurred at doses higher than 10 cGy.

Original languageEnglish
Pages (from-to)197-205
Number of pages9
JournalJournal of Neuroscience Methods
Issue number2
StatePublished - Apr 15 2003


  • Explant
  • High-LET
  • Image analysis
  • Neurite outgrowth quantification
  • Neurotoxicity
  • Retinal ganglion


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