Alternative methods of extracting telomerase activity from human tumor samples

Lauren S. Gollahon, Shawn E. Holt

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Telomerase activity is present in approximately 85% of malignant cancers and >30% of premalignant lesions. Extraction of telomerase from solid tumors and tissues remains difficult and inconvenient due to the presence of PCR inhibitors. Here we show that PCR inhibitors are easily removed during an initial extraction, allowing detection of telomerase activity in subsequent extractions from the same sample. In addition, telomerase activity may be enriched and detected from very small samples in a large background by utilizing a biotin/strept-avidin coated, magnetic bead retrieval assay. Our results provide alternative methods for telomerase extraction from solid tumors and small samples that are more convenient and accurate. (C) 2000 Published by Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalCancer Letters
Issue number2
StatePublished - Oct 31 2000


  • Enhanced telomerase extraction
  • Telomere repeat amplification protocol
  • Tumor samples


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