Alternative methods of extracting telomerase activity from human tumor samples

Lauren S. Gollahon, Shawn E. Holt

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Telomerase activity is present in approximately 85% of malignant cancers and >30% of premalignant lesions. Extraction of telomerase from solid tumors and tissues remains difficult and inconvenient due to the presence of PCR inhibitors. Here we show that PCR inhibitors are easily removed during an initial extraction, allowing detection of telomerase activity in subsequent extractions from the same sample. In addition, telomerase activity may be enriched and detected from very small samples in a large background by utilizing a biotin/strept-avidin coated, magnetic bead retrieval assay. Our results provide alternative methods for telomerase extraction from solid tumors and small samples that are more convenient and accurate. (C) 2000 Published by Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalCancer Letters
Volume159
Issue number2
DOIs
StatePublished - Oct 31 2000

Keywords

  • Enhanced telomerase extraction
  • Telomere repeat amplification protocol
  • Tumor samples

Fingerprint Dive into the research topics of 'Alternative methods of extracting telomerase activity from human tumor samples'. Together they form a unique fingerprint.

  • Cite this