Albumin inhibits Pseudomonas aeruginosa quorum sensing and alters polymicrobial interactions

Allie Clinton Smith, Anne Rice, Bryan Sutton, Rebecca Gabrilska, Aimee K. Wessel, Marvin Whiteley, Kendra P. Rumbaugh

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Polymicrobial interactions are complex and can influence the course of an infection, as is the case when two or more species exhibit a synergism that produces a disease state not seen with any of the individual species alone. Cell-to-cell signaling is key to many of these interactions, but little is understood about how the host environment influences polymicrobial interactions or signaling between bacteria. Chronic wounds are typically polymicrobial, with Staphylococcus aureus and Pseudomonas aeruginosa being the two most commonly isolated species. While P. aeruginosa readily kills S. aureus in vitro, the two species can coexist for long periods together in chronic wound infections. In this study, we investigated the ability of components of the wound environment to modulate interactions between P. aeruginosa and S. aureus. We demonstrate that P. aeruginosa quorum sensing is inhibited by physiological levels of serum albumin, which appears to bind and sequester some homoserine lactone quorum signals, resulting in the inability of P. aeruginosa to produce virulence factors that kill S. aureus. These data could provide important clues regarding the virulence of P. aeruginosa in albumin-depleted versus albuminrich infection sites and an understanding of the nature of friendly versus antagonistic interactions between P. aeruginosa and S. aureus.

Original languageEnglish
Article numbere00116-17
JournalInfection and Immunity
Volume85
Issue number9
DOIs
StatePublished - Sep 1 2017

Keywords

  • Albumin
  • Polymicrobial infection
  • Pseudomonas aeruginosa
  • Quorum sensing
  • Staphylococcus aureus
  • Wound

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    Smith, A. C., Rice, A., Sutton, B., Gabrilska, R., Wessel, A. K., Whiteley, M., & Rumbaugh, K. P. (2017). Albumin inhibits Pseudomonas aeruginosa quorum sensing and alters polymicrobial interactions. Infection and Immunity, 85(9), [e00116-17]. https://doi.org/10.1128/IAI.00116-17