TY - JOUR
T1 - AKR-deficiency disturbs the balance of some signal transduction pathways in Arabidopsis thaliana
AU - Wang, Jing
AU - Yan, Juqiang
AU - Zhang, Hong
N1 - Funding Information:
We thank Drs Frederick Ausubel, Howard Goodman, Guoliang Yu, Hong Ma, Brenda Shirley for DNA probes used in this experiment, and we thank Allison Richey for critically reading the manuscript. We are grateful to Dr Howard Goodman for his generous support of this project. This work was partially supported by a grant from the Texas Advanced Technology program.
PY - 1999/6
Y1 - 1999/6
N2 - High-intensity light induces anthocyanin production in wild-type Arabidopsis leaves, but this induction is largely abolished in the chlorotic leaf tissues of AKR (ankyrin repeat-containing protein)-deficient plants. The steady-state mRNA levels of three anthocyanin biosynthetic genes, CHI, CHS and DFR, did not increase in response to high-intensity light treatment in chlorotic leaf tissues, whereas they increased several fold in green leaf tissues. There is a good correlation between anthocyanin production and transcript levels of anthocyanin biosynthetic genes, especially DFR, in green leaf tissues. In contrast, the transcripts of TCH2 and TCH3 that encode for calmodulin-related proteins and GPA that encodes for the α subunit of the trimeric G protein were much higher in chlorotic leaf tissues than those in green leaf tissues. These data suggest that the AKR-deficiency could increase gene expression in one signal transduction pathway and at the same time repress gene expression in another signal transduction pathway, which alludes to a possible mechanism for AKR involvement in chloroplast development.
AB - High-intensity light induces anthocyanin production in wild-type Arabidopsis leaves, but this induction is largely abolished in the chlorotic leaf tissues of AKR (ankyrin repeat-containing protein)-deficient plants. The steady-state mRNA levels of three anthocyanin biosynthetic genes, CHI, CHS and DFR, did not increase in response to high-intensity light treatment in chlorotic leaf tissues, whereas they increased several fold in green leaf tissues. There is a good correlation between anthocyanin production and transcript levels of anthocyanin biosynthetic genes, especially DFR, in green leaf tissues. In contrast, the transcripts of TCH2 and TCH3 that encode for calmodulin-related proteins and GPA that encodes for the α subunit of the trimeric G protein were much higher in chlorotic leaf tissues than those in green leaf tissues. These data suggest that the AKR-deficiency could increase gene expression in one signal transduction pathway and at the same time repress gene expression in another signal transduction pathway, which alludes to a possible mechanism for AKR involvement in chloroplast development.
KW - Anthocyanin
KW - Arabidopsis thaliana
KW - Chlorosis
KW - High-intensity light
KW - Signal transduction
UR - http://www.scopus.com/inward/record.url?scp=0032775913&partnerID=8YFLogxK
U2 - 10.1016/S0981-9428(99)80051-5
DO - 10.1016/S0981-9428(99)80051-5
M3 - Article
AN - SCOPUS:0032775913
SN - 0981-9428
VL - 37
SP - 465
EP - 471
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
IS - 6
ER -