Agrobacterium-mediated transformation of tomatillo (Physalis ixocarpa) and tissue specific and developmental expression of the CaMV 35S promoter in transgenic tomatillo plants

Nacyra Assad-García; Neftali Ochoa-Alejo; Enrique García-Hernández; Luis Herrera-Estrella; June Simpson

doi:10.1007/BF00233092

Agrobacterium-mediated transformation of tomatillo (Physalis ixocarpa) and tissue specific and developmental expression of the CaMV 35S promoter in transgenic tomatillo plants

Nacyra Assad-García, Neftali Ochoa-Alejo, Enrique García-Hernández, Luis Herrera-Estrella, June Simpson

Plant & Soil Science

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A protocol for the Agrobacterium-mediated transformation of tomatillo was developed. Up to 40 transgenic plants could be obtained in experiments using 60 cotyledon expiants. The transformed nature of the regenerated plants was confirmed by NPT II and Southern blot hybridization analysis. Using the b-glucuronidase system the tissue specific and developmental patterns of expression of the Cauliflower Mosaic Virus 35S promoter were determined in transgenic tomatillo plants. It was found that this promoter is developmentally regulated during fruit and seed formation.

Original language	English
Pages (from-to)	558-562
Number of pages	5
Journal	Plant Cell Reports
Volume	11
Issue number	11
DOIs	https://doi.org/10.1007/BF00233092
State	Published - Oct 1992

Keywords

Agrobacterium
CaMV 35S Promoter
Gene Expression
Physalis ixocarpa
Plant Transformation

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10.1007/BF00233092

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@article{0e72359e20e34563aa76d2fb9783389a,

title = "Agrobacterium-mediated transformation of tomatillo (Physalis ixocarpa) and tissue specific and developmental expression of the CaMV 35S promoter in transgenic tomatillo plants",

abstract = "A protocol for the Agrobacterium-mediated transformation of tomatillo was developed. Up to 40 transgenic plants could be obtained in experiments using 60 cotyledon expiants. The transformed nature of the regenerated plants was confirmed by NPT II and Southern blot hybridization analysis. Using the b-glucuronidase system the tissue specific and developmental patterns of expression of the Cauliflower Mosaic Virus 35S promoter were determined in transgenic tomatillo plants. It was found that this promoter is developmentally regulated during fruit and seed formation.",

keywords = "Agrobacterium, CaMV 35S Promoter, Gene Expression, Physalis ixocarpa, Plant Transformation",

author = "Nacyra Assad-Garc{\'i}a and Neftali Ochoa-Alejo and Enrique Garc{\'i}a-Hern{\'a}ndez and Luis Herrera-Estrella and June Simpson",

year = "1992",

month = oct,

doi = "10.1007/BF00233092",

language = "English",

volume = "11",

pages = "558--562",

journal = "Plant Cell Reports",

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number = "11",

}

Agrobacterium-mediated transformation of tomatillo (Physalis ixocarpa) and tissue specific and developmental expression of the CaMV 35S promoter in transgenic tomatillo plants. / Assad-García, Nacyra; Ochoa-Alejo, Neftali; García-Hernández, Enrique; Herrera-Estrella, Luis; Simpson, June.

In: Plant Cell Reports, Vol. 11, No. 11, 10.1992, p. 558-562.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Agrobacterium-mediated transformation of tomatillo (Physalis ixocarpa) and tissue specific and developmental expression of the CaMV 35S promoter in transgenic tomatillo plants

AU - Assad-García, Nacyra

AU - Ochoa-Alejo, Neftali

AU - García-Hernández, Enrique

AU - Herrera-Estrella, Luis

AU - Simpson, June

PY - 1992/10

Y1 - 1992/10

N2 - A protocol for the Agrobacterium-mediated transformation of tomatillo was developed. Up to 40 transgenic plants could be obtained in experiments using 60 cotyledon expiants. The transformed nature of the regenerated plants was confirmed by NPT II and Southern blot hybridization analysis. Using the b-glucuronidase system the tissue specific and developmental patterns of expression of the Cauliflower Mosaic Virus 35S promoter were determined in transgenic tomatillo plants. It was found that this promoter is developmentally regulated during fruit and seed formation.

AB - A protocol for the Agrobacterium-mediated transformation of tomatillo was developed. Up to 40 transgenic plants could be obtained in experiments using 60 cotyledon expiants. The transformed nature of the regenerated plants was confirmed by NPT II and Southern blot hybridization analysis. Using the b-glucuronidase system the tissue specific and developmental patterns of expression of the Cauliflower Mosaic Virus 35S promoter were determined in transgenic tomatillo plants. It was found that this promoter is developmentally regulated during fruit and seed formation.

KW - Agrobacterium

KW - CaMV 35S Promoter

KW - Gene Expression

KW - Physalis ixocarpa

KW - Plant Transformation

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VL - 11

SP - 558

EP - 562

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 11

ER -

Agrobacterium-mediated transformation of tomatillo (Physalis ixocarpa) and tissue specific and developmental expression of the CaMV 35S promoter in transgenic tomatillo plants

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Keywords

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