Agrobacterium-mediated transformation of tomatillo (Physalis ixocarpa) and tissue specific and developmental expression of the CaMV 35S promoter in transgenic tomatillo plants

Nacyra Assad-García, Neftali Ochoa-Alejo, Enrique García-Hernández, Luis Herrera-Estrella, June Simpson

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A protocol for the Agrobacterium-mediated transformation of tomatillo was developed. Up to 40 transgenic plants could be obtained in experiments using 60 cotyledon expiants. The transformed nature of the regenerated plants was confirmed by NPT II and Southern blot hybridization analysis. Using the b-glucuronidase system the tissue specific and developmental patterns of expression of the Cauliflower Mosaic Virus 35S promoter were determined in transgenic tomatillo plants. It was found that this promoter is developmentally regulated during fruit and seed formation.

Original languageEnglish
Pages (from-to)558-562
Number of pages5
JournalPlant Cell Reports
Volume11
Issue number11
DOIs
StatePublished - Oct 1992

Keywords

  • Agrobacterium
  • CaMV 35S Promoter
  • Gene Expression
  • Physalis ixocarpa
  • Plant Transformation

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