TY - JOUR
T1 - Agrobacterium-mediated transformation of Amaranthus hypochondriacus
T2 - Light, and tissue-specific expression of a pea chlorophyll a/b-binding protein promoter
AU - Jofre-Garfias, A. E.
AU - Villegas-Sepúlveda, N.
AU - Cabrera-Ponce, J. L.
AU - Adame-Alvarez, R. M.
AU - Herrera-Estrella, L.
AU - Simpson, J.
PY - 1997
Y1 - 1997
N2 - Mature embryos of Amaranthus hypochondriacus (amaranth) were used to develop an in vitro culture system for plant regeneration and genetic transformation. Plants were regenerated from embryo-derived callus cultivated on Murashige and Skoog medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid or 3,6-dichloro-2-methoxybenzoic acid and 10% coconut liquid endosperm. Transgenic plants were obtained by inoculation of mature embryo explants with a disarmed Agrobacterium strain containing the plasmid pGV2260(pEsc4), which carried the genes encoding neomycin phosphotransferase type II and β-glucuronidase. The presence of transgenes in the genome of transformed amaranth plants and their progeny was demonstrated by Southern blot hybridization. Tissue specific and light-inducible expression directed by a pea chlorophyll a/b-binding protein promoter was observed in transgenic amaranth plants and their progeny.
AB - Mature embryos of Amaranthus hypochondriacus (amaranth) were used to develop an in vitro culture system for plant regeneration and genetic transformation. Plants were regenerated from embryo-derived callus cultivated on Murashige and Skoog medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid or 3,6-dichloro-2-methoxybenzoic acid and 10% coconut liquid endosperm. Transgenic plants were obtained by inoculation of mature embryo explants with a disarmed Agrobacterium strain containing the plasmid pGV2260(pEsc4), which carried the genes encoding neomycin phosphotransferase type II and β-glucuronidase. The presence of transgenes in the genome of transformed amaranth plants and their progeny was demonstrated by Southern blot hybridization. Tissue specific and light-inducible expression directed by a pea chlorophyll a/b-binding protein promoter was observed in transgenic amaranth plants and their progeny.
KW - Agrobacterium tumefaciens
KW - Amaranthus hypochondriacus
KW - Genetic transformation
KW - Light regulation
KW - NPT II
KW - β-Glucuronidase
UR - http://www.scopus.com/inward/record.url?scp=1842301051&partnerID=8YFLogxK
U2 - 10.1007/s002990050332
DO - 10.1007/s002990050332
M3 - Article
AN - SCOPUS:1842301051
SN - 0721-7714
VL - 16
SP - 847
EP - 852
JO - Plant Cell Reports
JF - Plant Cell Reports
IS - 12
ER -