Agrobacterium-mediated transformation of Amaranthus hypochondriacus: Light, and tissue-specific expression of a pea chlorophyll a/b-binding protein promoter

A. E. Jofre-Garfias, N. Villegas-Sepúlveda, J. L. Cabrera-Ponce, R. M. Adame-Alvarez, L. Herrera-Estrella, J. Simpson

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Mature embryos of Amaranthus hypochondriacus (amaranth) were used to develop an in vitro culture system for plant regeneration and genetic transformation. Plants were regenerated from embryo-derived callus cultivated on Murashige and Skoog medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid or 3,6-dichloro-2-methoxybenzoic acid and 10% coconut liquid endosperm. Transgenic plants were obtained by inoculation of mature embryo explants with a disarmed Agrobacterium strain containing the plasmid pGV2260(pEsc4), which carried the genes encoding neomycin phosphotransferase type II and β-glucuronidase. The presence of transgenes in the genome of transformed amaranth plants and their progeny was demonstrated by Southern blot hybridization. Tissue specific and light-inducible expression directed by a pea chlorophyll a/b-binding protein promoter was observed in transgenic amaranth plants and their progeny.

Original languageEnglish
Pages (from-to)847-852
Number of pages6
JournalPlant Cell Reports
Volume16
Issue number12
DOIs
StatePublished - 1997

Keywords

  • Agrobacterium tumefaciens
  • Amaranthus hypochondriacus
  • Genetic transformation
  • Light regulation
  • NPT II
  • β-Glucuronidase

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