TY - JOUR
T1 - Aerobic training but no resistance training increases SIRT3 in skeletal muscle of sedentary obese male adolescents
AU - Vargas-Ortiz, Katya
AU - Pérez-Vázquez, Victoriano
AU - Figueroa, Arturo
AU - Díaz, Francisco J.
AU - Montaño-Ascencio, Paulina G.
AU - Macías-Cervantes, Maciste H.
N1 - Funding Information:
This work was supported by University of Guanajuato under grant [EC4F02112]. Katya Vargas-Ortiz had CONACYT scholarship [No. 328312]. We appreciate the support by Nutritionist Alejandra Campos-Cervantes.
Publisher Copyright:
© 2017 European College of Sport Science.
PY - 2018/2/7
Y1 - 2018/2/7
N2 - In recent years, prevalence of obesity in children and adolescents has increased. A strategy for prevention and management of obesity is aerobic training (AT) due to its effectiveness to decrease fat mass. AT increases the content of SIRT3, a mitochondrial protein that increases the expression of PGC-1α and NFR1, thereby enhances mitochondrial function and metabolic health. Resistance training (RT) provides metabolic benefits but its effect on SIRT3 content is unknown. To compare the effect of AT and RT on SIRT3, PGC-1α and NRF-1 protein levels in skeletal muscle of sedentary obese adolescents. Twenty-seven sedentary obese male adolescents (age: 16.7 ± 0.9 years; BMI: 33.7 ± 4.3 kg/m2) completed a 1-month control period prior to randomization to one of two supervised exercise protocols: AT (3 days/week, 40 min/day, 70–80% peak heart rate) or RT (3 days/week, 11 exercises, 2 sets/exercise, 12 repetitions/set) for 12 weeks. Biopsies were obtained from the vastus lateralis muscle before and after 12 weeks to analyse SIRT3, PGC-1α and NRF-1 proteins content. Peak oxygen consumption (VO2peak) and anthropometric variables were evaluated before and after training. AT increased SIRT3 content, which was associated with improvements in PGC-1α content and body fat percentage. RT did not affect SIRT3 or PGC-1α. VO2peak increased only in AT. The increase in muscle mitochondrial SIRT3 was observed only following AT. In contrast, RT increased muscle mass without improving SIRT3 in obese male adolescents.
AB - In recent years, prevalence of obesity in children and adolescents has increased. A strategy for prevention and management of obesity is aerobic training (AT) due to its effectiveness to decrease fat mass. AT increases the content of SIRT3, a mitochondrial protein that increases the expression of PGC-1α and NFR1, thereby enhances mitochondrial function and metabolic health. Resistance training (RT) provides metabolic benefits but its effect on SIRT3 content is unknown. To compare the effect of AT and RT on SIRT3, PGC-1α and NRF-1 protein levels in skeletal muscle of sedentary obese adolescents. Twenty-seven sedentary obese male adolescents (age: 16.7 ± 0.9 years; BMI: 33.7 ± 4.3 kg/m2) completed a 1-month control period prior to randomization to one of two supervised exercise protocols: AT (3 days/week, 40 min/day, 70–80% peak heart rate) or RT (3 days/week, 11 exercises, 2 sets/exercise, 12 repetitions/set) for 12 weeks. Biopsies were obtained from the vastus lateralis muscle before and after 12 weeks to analyse SIRT3, PGC-1α and NRF-1 proteins content. Peak oxygen consumption (VO2peak) and anthropometric variables were evaluated before and after training. AT increased SIRT3 content, which was associated with improvements in PGC-1α content and body fat percentage. RT did not affect SIRT3 or PGC-1α. VO2peak increased only in AT. The increase in muscle mitochondrial SIRT3 was observed only following AT. In contrast, RT increased muscle mass without improving SIRT3 in obese male adolescents.
KW - Children
KW - body composition
KW - metabolism
KW - obesity
KW - sedentary living
UR - http://www.scopus.com/inward/record.url?scp=85043500091&partnerID=8YFLogxK
U2 - 10.1080/17461391.2017.1406007
DO - 10.1080/17461391.2017.1406007
M3 - Article
C2 - 29220206
AN - SCOPUS:85043500091
VL - 18
SP - 226
EP - 234
JO - European Journal of Sport Science
JF - European Journal of Sport Science
SN - 1746-1391
IS - 2
ER -