Advances in mass spectrometry-based glycoproteomics

Aiying Yu, Jingfu Zhao, Wenjing Peng, Alireza Banazadeh, Seth D. Williamson, Mona Goli, Yifan Huang, Yehia Mechref

Research output: Contribution to journalReview articlepeer-review

68 Scopus citations


Protein glycosylation, an important PTM, plays an essential role in a wide range of biological processes such as immune response, intercellular signaling, inflammation, and host–pathogen interaction. Aberrant glycosylation has been correlated with various diseases. However, studying protein glycosylation remains challenging because of low abundance, microheterogeneities of glycosylation sites, and poor ionization efficiency of glycopeptides. Therefore, the development of sensitive and accurate approaches to characterize protein glycosylation is crucial. The identification and characterization of protein glycosylation by MS is referred to as the field of glycoproteomics. Methods such as enrichment, metabolic labeling, and derivatization of glycopeptides in conjunction with different MS techniques and bioinformatics tools, have been developed to achieve an unequivocal quantitative and qualitative characterization of glycoproteins. This review summarizes the recent developments in the field of glycoproteomics over the past 6 years (2012 to 2018).

Original languageEnglish
Pages (from-to)3104-3122
Number of pages19
Issue number24
StatePublished - Dec 2018


  • Derivatization
  • Enrichment
  • Glycoproteins
  • Glycosylation
  • Metabolic labeling


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