Advances in mass spectrometry-based glycomics

Xue Dong, Yifan Huang, Byeong Gwan Cho, Jieqiang Zhong, Sakshi Gautam, Wenjing Peng, Seth D. Williamson, Alireza Banazadeh, Katya Y. Torres-Ulloa, Yehia Mechref

Research output: Contribution to journalReview article

16 Scopus citations

Abstract

The diversification of the chemical properties and biological functions of proteins is attained through posttranslational modifications, such as glycosylation. Glycans, which are covalently attached to proteins, play a vital role in cell activities. The microheterogeneity and complexity of glycan structures associated with proteins make comprehensive glycomic analysis challenging. However, recent advancements in mass spectrometry (MS), separation techniques, and sample preparation methods have primarily facilitated structural elucidation and quantitation of glycans. This review focuses on describing recent advances in MS-based techniques used for glycomic analysis (2012–2018), including ionization, tandem MS, and separation techniques coupled with MS. Progress in glycomics workflow involving glycan release, purification, derivatization, and separation will also be highlighted here. Additionally, the recent development of quantitative glycomics through comparative and multiplex approaches will also be described.

Original languageEnglish
Pages (from-to)3063-3081
Number of pages19
JournalELECTROPHORESIS
Volume39
Issue number24
DOIs
StatePublished - Dec 2018

Keywords

  • LC–MS/MS
  • glycan
  • isomeric separation
  • liquid chromatography
  • mass spectrometry
  • quantitative analysis

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    Dong, X., Huang, Y., Cho, B. G., Zhong, J., Gautam, S., Peng, W., Williamson, S. D., Banazadeh, A., Torres-Ulloa, K. Y., & Mechref, Y. (2018). Advances in mass spectrometry-based glycomics. ELECTROPHORESIS, 39(24), 3063-3081. https://doi.org/10.1002/elps.201800273