TY - JOUR
T1 - Advances in mass spectrometry-based glycomics
AU - Dong, Xue
AU - Huang, Yifan
AU - Cho, Byeong Gwan
AU - Zhong, Jieqiang
AU - Gautam, Sakshi
AU - Peng, Wenjing
AU - Williamson, Seth D.
AU - Banazadeh, Alireza
AU - Torres-Ulloa, Katya Y.
AU - Mechref, Yehia
N1 - Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/12
Y1 - 2018/12
N2 - The diversification of the chemical properties and biological functions of proteins is attained through posttranslational modifications, such as glycosylation. Glycans, which are covalently attached to proteins, play a vital role in cell activities. The microheterogeneity and complexity of glycan structures associated with proteins make comprehensive glycomic analysis challenging. However, recent advancements in mass spectrometry (MS), separation techniques, and sample preparation methods have primarily facilitated structural elucidation and quantitation of glycans. This review focuses on describing recent advances in MS-based techniques used for glycomic analysis (2012–2018), including ionization, tandem MS, and separation techniques coupled with MS. Progress in glycomics workflow involving glycan release, purification, derivatization, and separation will also be highlighted here. Additionally, the recent development of quantitative glycomics through comparative and multiplex approaches will also be described.
AB - The diversification of the chemical properties and biological functions of proteins is attained through posttranslational modifications, such as glycosylation. Glycans, which are covalently attached to proteins, play a vital role in cell activities. The microheterogeneity and complexity of glycan structures associated with proteins make comprehensive glycomic analysis challenging. However, recent advancements in mass spectrometry (MS), separation techniques, and sample preparation methods have primarily facilitated structural elucidation and quantitation of glycans. This review focuses on describing recent advances in MS-based techniques used for glycomic analysis (2012–2018), including ionization, tandem MS, and separation techniques coupled with MS. Progress in glycomics workflow involving glycan release, purification, derivatization, and separation will also be highlighted here. Additionally, the recent development of quantitative glycomics through comparative and multiplex approaches will also be described.
KW - LC–MS/MS
KW - glycan
KW - isomeric separation
KW - liquid chromatography
KW - mass spectrometry
KW - quantitative analysis
UR - http://www.scopus.com/inward/record.url?scp=85054564985&partnerID=8YFLogxK
U2 - 10.1002/elps.201800273
DO - 10.1002/elps.201800273
M3 - Review article
C2 - 30199110
AN - SCOPUS:85054564985
SN - 0173-0835
VL - 39
SP - 3063
EP - 3081
JO - ELECTROPHORESIS
JF - ELECTROPHORESIS
IS - 24
ER -