Adipose tissue stearoyl-CoA desaturase mRNA is increased by obesity and decreased by polyunsaturated fatty acids

Brynn H. Jones, Margaret A. Mäher, William J. Banz, Michael B. Zemel, Jay Whelan, Pamela J. Smith, Naïma Moustaïd

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

-Stearovl-CoA desaturase (SCD) is a kev regulatory enzyme in the synthesis of unsaturated fatty acids. Although regulation of hepatic SCD by obesity and polyunsaturated fatty acids (PUFA) has been well investigated, no studies have addressed whether similar regulation occurs in adipose tissue. We addressed these questions by feeding control (12% corn oil) and high-PUFA (48% corn oil) diets to lean and obese Zucker rats and analyzing SCD mRNA levels in adipose tissue and liver. We report that SCD mRNA content was dramatically elevated in adipose tissue of obese vs. lean rats on both diets and was significantly decreased by PUFA in both genotypes. Interestingly, we demonstrate that SCD expression was directly downregulated in a dosedependent manner by PUFA in 3T3-L1 adipocytes. We conclude that 1 ) obese Zucker rats overexpress the SCD gene in both liver and adipose tissue and 2) PUFA directly suppress SCD expression in adipocytes. Further studies will elucidate the mechanisms responsible for obesity- and PUFA-mediated regulation of SCD in adipose cells.

Original languageEnglish
Pages (from-to)E50-E57
JournalAmerican Journal of Physiology
Volume271
Issue number1 PART 1
StatePublished - Dec 1 1996

    Fingerprint

Keywords

  • 3t3-l1 adipocytes
  • Linoleate
  • Zucker rats

Cite this

Jones, B. H., Mäher, M. A., Banz, W. J., Zemel, M. B., Whelan, J., Smith, P. J., & Moustaïd, N. (1996). Adipose tissue stearoyl-CoA desaturase mRNA is increased by obesity and decreased by polyunsaturated fatty acids. American Journal of Physiology, 271(1 PART 1), E50-E57.