Adipose Depot-Specific Differences in Transcriptome and MicroRNA Expression in High Fat Diet Induced Obese Mice

Nadeeja Wijayatunga, Mandana Pahlavani, Kameswara Kottapalli, John Dawson, Latha Ramalingam, Preethi H Gunaratne, Cristian Coarfa, Kimal Rajapakshe, Nishan S Kalupahana, Naima Moustaid-Moussa

Research output: Other contribution

Abstract

Obesity is characterized by white adipose tissue (WAT) expansion which contributes to chronic diseases such as diabetes and cardiovascular disease. Two main types of adipose tissue are WAT (subcutaneous, SAT and visceral adipose tissue, VAT) and brown adipose tissue (BAT). These exhibit anatomical, physiological and metabolic differences that contributes to energy balance, metabolic regulation and immunity. Our aim was to compare transcriptomic and microRNA (miRNA) signatures of SAT, VAT and BAT to gain mechanistic insight into the role of these depots in metabolic disorders. We performed RNA-sequencing and miRNA profiling of SAT, VAT and BAT depots from diet-induced obese C57BL/6J male mice fed high fat diets (45% kcal fat). We used a transcriptomic approach to dissect adipose depot-specific genetic and functional signatures in obesity. RNA sequencing was performed using Illumina Hi-Seq and differential gene expression was studied by pair wise comparisons of fat depots. Functional an
Original languageEnglish
PublisherThe FASEB Journal
Volume30
StatePublished - 2016

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