Fluorescence and infrared spectroscopy and cholesterol oxidase activity were employed to investigate the effect of phosphatidylcholine (PC) acyl chain length mismatch on the lateral organizations of lipids in liquidordered dipalmitoyl-PC/dilauroyl-PC/cholesterol (DPPC/DLPC/CHOL) bilayers. Plots of steady-state fluorescence emission anisotropy of diphenylhexatriene (DPH) labeled PC (DPH-PC) embedded in the DPPC/ DLPC/CHOL bilayers revealed significant peaks at several DPPC mole fractions (YDPPC) when the cholesterol mole fraction (XCHOL) was fixed to particular values. Analogously, the DPH-PC anisotropy peaked at several critical XCHOL’s when YDPPC was fixed. Acyl chain C-H and CdO vibrational peak frequencies of native PC as well as the activity of cholesterol oxidase also revealed dips and peaks at similar YDPPC’s. Importantly, most of the observed peaks/dips coincide with the critical mole fractions predicted by the Superlattice (SL) model. A three-dimensional map of DPH-PC anisotro
|Journal||J. Chem. Phys. B|
|State||Published - Oct 2010|
Cannon, B., Lewis, A., Somerharju, P., Virtanen, J., Huang, J., & Cheng, K. (2010). Acyl-Chain Mismatch Driven Superlattice Arrangements in DPPC/DLPC/Cholesterol Bilayers. J. Chem. Phys. B, 10105-10113.