A simple sample preparation for enhancing the sensitivity of mass spectrometric oligosaccharide determinations through the use of an adsorptive hydrophobic resin

Y. Huang; Y. Mechref; J. Tian; H. Gong; W. J. Lennarz; M. V. Novotny

doi:10.1002/1097-0231(20000730)14:14<1233::AID-RCM16>3.0.CO;2-P

A simple sample preparation for enhancing the sensitivity of mass spectrometric oligosaccharide determinations through the use of an adsorptive hydrophobic resin

Y. Huang, Y. Mechref, J. Tian, H. Gong, W. J. Lennarz, M. V. Novotny

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A simple microadsorption technique is described to remove detergent additives from oligosaccharide samples before their mass spectrometric analysis. The described methodology has been validated with submicrogram quantities of contaminated glycoproteins. This procedure is applicable to investigating minute quantities of glycans in both the positive- and negative-ion mode of matrix-assisted laser desorption/ionization mass spectrometry. (C) 2000 John Wiley and Sons, Ltd.

Original language	English
Pages (from-to)	1233-1237
Number of pages	5
Journal	Rapid Communications in Mass Spectrometry
Volume	14
Issue number	14
DOIs	https://doi.org/10.1002/1097-0231(20000730)14:14<1233::AID-RCM16>3.0.CO;2-P
State	Published - 2000

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10.1002/1097-0231(20000730)14:14<1233::AID-RCM16>3.0.CO;2-P

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Huang, Y., Mechref, Y., Tian, J., Gong, H., Lennarz, W. J., & Novotny, M. V. (2000). A simple sample preparation for enhancing the sensitivity of mass spectrometric oligosaccharide determinations through the use of an adsorptive hydrophobic resin. Rapid Communications in Mass Spectrometry, 14(14), 1233-1237. https://doi.org/10.1002/1097-0231(20000730)14:14<1233::AID-RCM16>3.0.CO;2-P

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Huang, Y, Mechref, Y, Tian, J, Gong, H, Lennarz, WJ & Novotny, MV 2000, 'A simple sample preparation for enhancing the sensitivity of mass spectrometric oligosaccharide determinations through the use of an adsorptive hydrophobic resin', Rapid Communications in Mass Spectrometry, vol. 14, no. 14, pp. 1233-1237. https://doi.org/10.1002/1097-0231(20000730)14:14<1233::AID-RCM16>3.0.CO;2-P

TY - JOUR

T1 - A simple sample preparation for enhancing the sensitivity of mass spectrometric oligosaccharide determinations through the use of an adsorptive hydrophobic resin

AU - Huang, Y.

AU - Mechref, Y.

AU - Tian, J.

AU - Gong, H.

AU - Lennarz, W. J.

AU - Novotny, M. V.

PY - 2000

Y1 - 2000

N2 - A simple microadsorption technique is described to remove detergent additives from oligosaccharide samples before their mass spectrometric analysis. The described methodology has been validated with submicrogram quantities of contaminated glycoproteins. This procedure is applicable to investigating minute quantities of glycans in both the positive- and negative-ion mode of matrix-assisted laser desorption/ionization mass spectrometry. (C) 2000 John Wiley and Sons, Ltd.

AB - A simple microadsorption technique is described to remove detergent additives from oligosaccharide samples before their mass spectrometric analysis. The described methodology has been validated with submicrogram quantities of contaminated glycoproteins. This procedure is applicable to investigating minute quantities of glycans in both the positive- and negative-ion mode of matrix-assisted laser desorption/ionization mass spectrometry. (C) 2000 John Wiley and Sons, Ltd.

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DO - 10.1002/1097-0231(20000730)14:14<1233::AID-RCM16>3.0.CO;2-P

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IS - 14

ER -

A simple sample preparation for enhancing the sensitivity of mass spectrometric oligosaccharide determinations through the use of an adsorptive hydrophobic resin

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