A quantitative investigation of fucosylated serum glycoproteins with application to esophageal adenocarcinoma

Benjamin Mann, Milan Madera, Iveta Klouckova, Yehia Mechref, Lacey E. Dobrolecki, Robert J. Hickey, Zane T. Hammoud, Milos V. Novotny

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Although glycoproteomic studies provide unique opportunities for cancer research, it has been necessary to develop specific methods for analysis of oncologically interesting glycoproteins. We describe a general, multimethodological approach for quantitative glycoproteomic analysis of fucosylated glycoproteins in human blood serum. A total of 136 putative fucosylated glycoproteins were identified with very high confidence in three clinically relevant sample pools (N = 5 for each), with a mean CV of 3.1% observed for replicate analyses. Two samples were collected from subjects diagnosed with esophagus disease states, high-grade dysplasia plus esophageal adenocarcinoma, while the third sample was representative of a disease-free condition. Some glycoproteins, observed to be significantly upregulated in esophageal adenocarcinoma, i.e. more than twofold higher than in the disease-free condition, are briefly discussed. Further investigation will be necessary to validate these findings; however, the method itself is demonstrated to be an effective tool for quantitative glycoproteomics of clinical samples.

Original languageEnglish
Pages (from-to)1833-1841
Number of pages9
JournalELECTROPHORESIS
Volume31
Issue number11
DOIs
StatePublished - Jun 2010

Keywords

  • Esophageal adenocarcinoma
  • Fucosylation
  • Glycoproteomics
  • Lectin affinity
  • Quantitative analysis

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