A new highly effective anticysticercosis vaccine expressed in transgenic papaya

Marisela Hernández; José Luis Cabrera-Ponce; Gladis Fragoso; Fernando López-Casillas; Arturo Guevara-García; Gabriela Rosas; Claudia León-Ramírez; Patricia Juárez; Guadalupe Sánchez-García; Jaquelynne Cervantes; Gonzalo Acero; Andrea Toledo; Carmen Cruz; Rafael Bojalil; Luis Herrera-Estrella; Edda Sciutto

doi:10.1016/j.vaccine.2007.02.080

A new highly effective anticysticercosis vaccine expressed in transgenic papaya

Marisela Hernández, José Luis Cabrera-Ponce, Gladis Fragoso, Fernando López-Casillas, Arturo Guevara-García, Gabriela Rosas, Claudia León-Ramírez, Patricia Juárez, Guadalupe Sánchez-García, Jaquelynne Cervantes, Gonzalo Acero, Andrea Toledo, Carmen Cruz, Rafael Bojalil, Luis Herrera-Estrella, Edda Sciutto

Plant & Soil Science

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

The use of transgenic plants as new antigen-delivery systems for subunit vaccines has been increasingly explored. We herein report progress toward a papaya-based vaccine against cysticercosis. Synthetic peptides (KETc1, KETc12, KETc7) were successfully expressed in 19 different transgenic papaya clones and found to be immunogenic. Complete protection against cysticercosis was induced with the soluble extract of the clones that expressed the higher levels of transcripts in up to 90% of the immunized mice. This study represents a key step towards the development of a more effective, sustainable and affordable oral subunit vaccine against human and pig cysticercosis.

Original language	English
Pages (from-to)	4252-4260
Number of pages	9
Journal	Vaccine
Volume	25
Issue number	21
DOIs	https://doi.org/10.1016/j.vaccine.2007.02.080
State	Published - May 22 2007

Keywords

Anti-cysticercosis vaccine
Cysticercosis control
Transgenic papaya

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10.1016/j.vaccine.2007.02.080

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Hernández, M., Cabrera-Ponce, J. L., Fragoso, G., López-Casillas, F., Guevara-García, A., Rosas, G., León-Ramírez, C., Juárez, P., Sánchez-García, G., Cervantes, J., Acero, G., Toledo, A., Cruz, C., Bojalil, R., Herrera-Estrella, L., & Sciutto, E. (2007). A new highly effective anticysticercosis vaccine expressed in transgenic papaya. Vaccine, 25(21), 4252-4260. https://doi.org/10.1016/j.vaccine.2007.02.080

Hernández, Marisela ; Cabrera-Ponce, José Luis ; Fragoso, Gladis ; López-Casillas, Fernando ; Guevara-García, Arturo ; Rosas, Gabriela ; León-Ramírez, Claudia ; Juárez, Patricia ; Sánchez-García, Guadalupe ; Cervantes, Jaquelynne ; Acero, Gonzalo ; Toledo, Andrea ; Cruz, Carmen ; Bojalil, Rafael ; Herrera-Estrella, Luis ; Sciutto, Edda. / A new highly effective anticysticercosis vaccine expressed in transgenic papaya. In: Vaccine. 2007 ; Vol. 25, No. 21. pp. 4252-4260.

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title = "A new highly effective anticysticercosis vaccine expressed in transgenic papaya",

abstract = "The use of transgenic plants as new antigen-delivery systems for subunit vaccines has been increasingly explored. We herein report progress toward a papaya-based vaccine against cysticercosis. Synthetic peptides (KETc1, KETc12, KETc7) were successfully expressed in 19 different transgenic papaya clones and found to be immunogenic. Complete protection against cysticercosis was induced with the soluble extract of the clones that expressed the higher levels of transcripts in up to 90% of the immunized mice. This study represents a key step towards the development of a more effective, sustainable and affordable oral subunit vaccine against human and pig cysticercosis.",

keywords = "Anti-cysticercosis vaccine, Cysticercosis control, Transgenic papaya",

author = "Marisela Hern{\'a}ndez and Cabrera-Ponce, {Jos{\'e} Luis} and Gladis Fragoso and Fernando L{\'o}pez-Casillas and Arturo Guevara-Garc{\'i}a and Gabriela Rosas and Claudia Le{\'o}n-Ram{\'i}rez and Patricia Ju{\'a}rez and Guadalupe S{\'a}nchez-Garc{\'i}a and Jaquelynne Cervantes and Gonzalo Acero and Andrea Toledo and Carmen Cruz and Rafael Bojalil and Luis Herrera-Estrella and Edda Sciutto",

note = "Funding Information: We acknowledge Valentin Mendoza, Alicia Chagoya, Elva Teresa Ar{\'e}chiga Carvajal, Sergio Casas, Gerardo Arrell{\'i}n, Beatriz Hern{\'a}ndez, Ver{\'o}nica Rodr{\'i}guez Mata, Salvador Guzm{\'a}n, Martha Lara, Mercedes Baca and Georgina Diaz for their technical support; Carlos Larralde for his useful comments; Isabel P{\'e}rez Montfort for English correction of this manuscript, and the PhD Degree Program in Biological Sciences at the Universidad Aut{\'o}noma Metropolitana, Unidad Xochimilco, Mexico City, where Marisela Hernandez studies her PhD degree. This research was partially supported by the CONACyT (46953-m; 2004-01-040), DGAPA (IN 221905) and The Howard Hughes Medical Institute (55004134).",

year = "2007",

month = may,

day = "22",

doi = "10.1016/j.vaccine.2007.02.080",

language = "English",

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Hernández, M, Cabrera-Ponce, JL, Fragoso, G, López-Casillas, F, Guevara-García, A, Rosas, G, León-Ramírez, C, Juárez, P, Sánchez-García, G, Cervantes, J, Acero, G, Toledo, A, Cruz, C, Bojalil, R, Herrera-Estrella, L & Sciutto, E 2007, 'A new highly effective anticysticercosis vaccine expressed in transgenic papaya', Vaccine, vol. 25, no. 21, pp. 4252-4260. https://doi.org/10.1016/j.vaccine.2007.02.080

A new highly effective anticysticercosis vaccine expressed in transgenic papaya. / Hernández, Marisela; Cabrera-Ponce, José Luis; Fragoso, Gladis; López-Casillas, Fernando; Guevara-García, Arturo; Rosas, Gabriela; León-Ramírez, Claudia; Juárez, Patricia; Sánchez-García, Guadalupe; Cervantes, Jaquelynne; Acero, Gonzalo; Toledo, Andrea; Cruz, Carmen; Bojalil, Rafael; Herrera-Estrella, Luis; Sciutto, Edda.

In: Vaccine, Vol. 25, No. 21, 22.05.2007, p. 4252-4260.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A new highly effective anticysticercosis vaccine expressed in transgenic papaya

AU - Hernández, Marisela

AU - Cabrera-Ponce, José Luis

AU - Fragoso, Gladis

AU - López-Casillas, Fernando

AU - Guevara-García, Arturo

AU - Rosas, Gabriela

AU - León-Ramírez, Claudia

AU - Juárez, Patricia

AU - Sánchez-García, Guadalupe

AU - Cervantes, Jaquelynne

AU - Acero, Gonzalo

AU - Toledo, Andrea

AU - Cruz, Carmen

AU - Bojalil, Rafael

AU - Herrera-Estrella, Luis

AU - Sciutto, Edda

N1 - Funding Information: We acknowledge Valentin Mendoza, Alicia Chagoya, Elva Teresa Aréchiga Carvajal, Sergio Casas, Gerardo Arrellín, Beatriz Hernández, Verónica Rodríguez Mata, Salvador Guzmán, Martha Lara, Mercedes Baca and Georgina Diaz for their technical support; Carlos Larralde for his useful comments; Isabel Pérez Montfort for English correction of this manuscript, and the PhD Degree Program in Biological Sciences at the Universidad Autónoma Metropolitana, Unidad Xochimilco, Mexico City, where Marisela Hernandez studies her PhD degree. This research was partially supported by the CONACyT (46953-m; 2004-01-040), DGAPA (IN 221905) and The Howard Hughes Medical Institute (55004134).

PY - 2007/5/22

Y1 - 2007/5/22

N2 - The use of transgenic plants as new antigen-delivery systems for subunit vaccines has been increasingly explored. We herein report progress toward a papaya-based vaccine against cysticercosis. Synthetic peptides (KETc1, KETc12, KETc7) were successfully expressed in 19 different transgenic papaya clones and found to be immunogenic. Complete protection against cysticercosis was induced with the soluble extract of the clones that expressed the higher levels of transcripts in up to 90% of the immunized mice. This study represents a key step towards the development of a more effective, sustainable and affordable oral subunit vaccine against human and pig cysticercosis.

AB - The use of transgenic plants as new antigen-delivery systems for subunit vaccines has been increasingly explored. We herein report progress toward a papaya-based vaccine against cysticercosis. Synthetic peptides (KETc1, KETc12, KETc7) were successfully expressed in 19 different transgenic papaya clones and found to be immunogenic. Complete protection against cysticercosis was induced with the soluble extract of the clones that expressed the higher levels of transcripts in up to 90% of the immunized mice. This study represents a key step towards the development of a more effective, sustainable and affordable oral subunit vaccine against human and pig cysticercosis.

KW - Anti-cysticercosis vaccine

KW - Cysticercosis control

KW - Transgenic papaya

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U2 - 10.1016/j.vaccine.2007.02.080

DO - 10.1016/j.vaccine.2007.02.080

M3 - Article

C2 - 17399859

AN - SCOPUS:34247384648

VL - 25

SP - 4252

EP - 4260

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 21

ER -

A new highly effective anticysticercosis vaccine expressed in transgenic papaya

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Keywords

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