TY - JOUR
T1 - A new highly effective anticysticercosis vaccine expressed in transgenic papaya
AU - Hernández, Marisela
AU - Cabrera-Ponce, José Luis
AU - Fragoso, Gladis
AU - López-Casillas, Fernando
AU - Guevara-García, Arturo
AU - Rosas, Gabriela
AU - León-Ramírez, Claudia
AU - Juárez, Patricia
AU - Sánchez-García, Guadalupe
AU - Cervantes, Jaquelynne
AU - Acero, Gonzalo
AU - Toledo, Andrea
AU - Cruz, Carmen
AU - Bojalil, Rafael
AU - Herrera-Estrella, Luis
AU - Sciutto, Edda
N1 - Funding Information:
We acknowledge Valentin Mendoza, Alicia Chagoya, Elva Teresa Aréchiga Carvajal, Sergio Casas, Gerardo Arrellín, Beatriz Hernández, Verónica Rodríguez Mata, Salvador Guzmán, Martha Lara, Mercedes Baca and Georgina Diaz for their technical support; Carlos Larralde for his useful comments; Isabel Pérez Montfort for English correction of this manuscript, and the PhD Degree Program in Biological Sciences at the Universidad Autónoma Metropolitana, Unidad Xochimilco, Mexico City, where Marisela Hernandez studies her PhD degree. This research was partially supported by the CONACyT (46953-m; 2004-01-040), DGAPA (IN 221905) and The Howard Hughes Medical Institute (55004134).
PY - 2007/5/22
Y1 - 2007/5/22
N2 - The use of transgenic plants as new antigen-delivery systems for subunit vaccines has been increasingly explored. We herein report progress toward a papaya-based vaccine against cysticercosis. Synthetic peptides (KETc1, KETc12, KETc7) were successfully expressed in 19 different transgenic papaya clones and found to be immunogenic. Complete protection against cysticercosis was induced with the soluble extract of the clones that expressed the higher levels of transcripts in up to 90% of the immunized mice. This study represents a key step towards the development of a more effective, sustainable and affordable oral subunit vaccine against human and pig cysticercosis.
AB - The use of transgenic plants as new antigen-delivery systems for subunit vaccines has been increasingly explored. We herein report progress toward a papaya-based vaccine against cysticercosis. Synthetic peptides (KETc1, KETc12, KETc7) were successfully expressed in 19 different transgenic papaya clones and found to be immunogenic. Complete protection against cysticercosis was induced with the soluble extract of the clones that expressed the higher levels of transcripts in up to 90% of the immunized mice. This study represents a key step towards the development of a more effective, sustainable and affordable oral subunit vaccine against human and pig cysticercosis.
KW - Anti-cysticercosis vaccine
KW - Cysticercosis control
KW - Transgenic papaya
UR - http://www.scopus.com/inward/record.url?scp=34247384648&partnerID=8YFLogxK
U2 - 10.1016/j.vaccine.2007.02.080
DO - 10.1016/j.vaccine.2007.02.080
M3 - Article
C2 - 17399859
AN - SCOPUS:34247384648
VL - 25
SP - 4252
EP - 4260
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 21
ER -