TY - JOUR
T1 - A microfluidic cardiac flow profile generator for studying the effect of shear stress on valvular endothelial cells
AU - Kim, Jungkyu
AU - Lee, Joohyung
AU - Estlack, Zachary
AU - Somaweera, Balapitiyage Jeewani Himali
AU - Wang, Xinmei
AU - Lacerda, Carla
PY - 2018/8/14
Y1 - 2018/8/14
N2 - To precisely investigate the mechanobiological responses of valvular endothelial cells, we developed a microfluidic flow profile generator using a pneumatically-actuated micropump consisting of microvalves of various sizes. By controlling the closing pressures and the actuation times of these microvalves, we modulated the magnitude and frequency of the shear stress to mimic mitral and aortic inflow profiles with frequencies in the range of 0.8–2 Hz and shear stresses up to 20 dyn cm−2. To demonstrate this flow profile generator, aortic inflow with an average of 5.9 dyn cm−2 shear stress at a frequency of 1.2 Hz with a Reynolds number of 2.75, a Womersley number of 0.27, and an oscillatory shear index (OSI) value of 0.2 was applied to porcine aortic valvular endothelial cells (PAVECs) for mechanobiological studies. The cell alignment, cell elongation, and alpha-smooth muscle actin (αSMA) expression of PAVECs under perfusion, steady flow, and aortic inflow conditions were analyzed to de
AB - To precisely investigate the mechanobiological responses of valvular endothelial cells, we developed a microfluidic flow profile generator using a pneumatically-actuated micropump consisting of microvalves of various sizes. By controlling the closing pressures and the actuation times of these microvalves, we modulated the magnitude and frequency of the shear stress to mimic mitral and aortic inflow profiles with frequencies in the range of 0.8–2 Hz and shear stresses up to 20 dyn cm−2. To demonstrate this flow profile generator, aortic inflow with an average of 5.9 dyn cm−2 shear stress at a frequency of 1.2 Hz with a Reynolds number of 2.75, a Womersley number of 0.27, and an oscillatory shear index (OSI) value of 0.2 was applied to porcine aortic valvular endothelial cells (PAVECs) for mechanobiological studies. The cell alignment, cell elongation, and alpha-smooth muscle actin (αSMA) expression of PAVECs under perfusion, steady flow, and aortic inflow conditions were analyzed to de
M3 - Article
SP - 2946
EP - 2954
JO - Lab Chip
JF - Lab Chip
ER -