TY - JOUR
T1 - A mathematical model of cytosolic calcium dynamics in human umbilical vein endothelial cells
AU - Wiesner, Theodore F.
AU - Berk, Bradford C.
AU - Nerem, Robert M.
PY - 1996/5
Y1 - 1996/5
N2 - Important among the responses of endothelial cells are cytosolic free calcium transients. These transients are mediated by several factors, including blood-borne agonists, extracellular calcium, and fluid-imposed shear forces. The transients are characterized by a rapid rise followed by a plateau phase. A base mathematical model is presented that reasonably reproduces the measured calcium transient in cultured human umbilical vein endothelial cells responding to thrombin. Kinetic equations for receptor activation and calcium mobilization comprise the model. A graded response of intracellular free calcium to increasing concentrations of agonist is predicted. Also predicted is the elevation of the peak value and the plateau level by steady nonspecific leak of calcium across the plasma membrane. The influences of capacitative calcium entry, calcium-induced calcium release, and buffering by cytosolic proteins are investigated parametrically. The model predicts significant depletion of cellular calcium in response to agonist stimulation.
AB - Important among the responses of endothelial cells are cytosolic free calcium transients. These transients are mediated by several factors, including blood-borne agonists, extracellular calcium, and fluid-imposed shear forces. The transients are characterized by a rapid rise followed by a plateau phase. A base mathematical model is presented that reasonably reproduces the measured calcium transient in cultured human umbilical vein endothelial cells responding to thrombin. Kinetic equations for receptor activation and calcium mobilization comprise the model. A graded response of intracellular free calcium to increasing concentrations of agonist is predicted. Also predicted is the elevation of the peak value and the plateau level by steady nonspecific leak of calcium across the plasma membrane. The influences of capacitative calcium entry, calcium-induced calcium release, and buffering by cytosolic proteins are investigated parametrically. The model predicts significant depletion of cellular calcium in response to agonist stimulation.
KW - calcium buffering
KW - calcium influx
KW - calcium-induced calcium release
KW - capacitative calcium entry
KW - thrombin
UR - http://www.scopus.com/inward/record.url?scp=0029939623&partnerID=8YFLogxK
U2 - 10.1152/ajpcell.1996.270.5.c1556
DO - 10.1152/ajpcell.1996.270.5.c1556
M3 - Article
C2 - 8967458
AN - SCOPUS:0029939623
SN - 0363-6143
VL - 270
SP - C1556-C1569
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
IS - 5 39-5
ER -