A High Fat/High Sugar Diet Alters the Gastrointestinal Metabolome in a Sex Dependent Manner

Ayland C Letsinger; Rani Menon; Anjushree R Iyer; Heather Vellers; Jorge Z Granados; Arul Jayaraman; J. Timothy Lightfoot

doi:10.3390/metabo10100421

A High Fat/High Sugar Diet Alters the Gastrointestinal Metabolome in a Sex Dependent Manner

Ayland C Letsinger, Rani Menon, Anjushree R Iyer, Heather Vellers, Jorge Z Granados, Arul Jayaraman, J. Timothy Lightfoot

Kinesiology and Sport Management

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The gut metabolome offers insight for identifying the source of diet related pathology. As such, the purpose of this study was to characterize alterations of the gut metabolome in female and male C57BL/6J mice randomly assigned to a standard "chow" diet (CHOW) or a high fat/high sugar diet (HFHS; 45% fat and 20% fructose drinking solution) for nine weeks. Cecal metabolites were extracted and an untargeted analysis via LC-MS/MS was performed. Partial Least Sums Discriminate Analysis (PLS-DA) presented significant differences between the two diet groups in a sex-dependent manner. Mann-Whitney U-tests revealed 2443 and 1669 features to be significantly different between diet groups in the females and males, respectively. The majority of altered metabolites were depleted within the cecum of the HFHS fed mice. Metabolic pathways associated with galactose metabolism, leukotriene metabolism, and androgen and estrogen biosynthesis and metabolism were differentially altered with an HFHS diet b

Original language	English
Article number	421
Pages (from-to)	1-10
Number of pages	10
Journal	Metabolites
Volume	10
Issue number	10
DOIs	https://doi.org/10.3390/metabo10100421
State	Published - Oct 20 2020

Keywords

Androgen
Cecum
Estrogen
Fructose
Inflammation
Metabolism
Neurotransmitter
Obesity
Western diet

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10.3390/metabo10100421

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title = "A High Fat/High Sugar Diet Alters the Gastrointestinal Metabolome in a Sex Dependent Manner",

abstract = "The gut metabolome offers insight for identifying the source of diet related pathology. As such, the purpose of this study was to characterize alterations of the gut metabolome in female and male C57BL/6J mice randomly assigned to a standard {"}chow{"} diet (CHOW) or a high fat/high sugar diet (HFHS; 45% fat and 20% fructose drinking solution) for nine weeks. Cecal metabolites were extracted and an untargeted analysis via LC-MS/MS was performed. Partial Least Sums Discriminate Analysis (PLS-DA) presented significant differences between the two diet groups in a sex-dependent manner. Mann-Whitney U-tests revealed 2443 and 1669 features to be significantly different between diet groups in the females and males, respectively. The majority of altered metabolites were depleted within the cecum of the HFHS fed mice. Metabolic pathways associated with galactose metabolism, leukotriene metabolism, and androgen and estrogen biosynthesis and metabolism were differentially altered with an HFHS diet b",

keywords = "Androgen, Cecum, Estrogen, Fructose, Inflammation, Metabolism, Neurotransmitter, Obesity, Western diet",

author = "Letsinger, {Ayland C} and Rani Menon and Iyer, {Anjushree R} and Heather Vellers and Granados, {Jorge Z} and Arul Jayaraman and Lightfoot, {J. Timothy}",

note = "Funding Information: Funding: This research was funded by the Omar Smith Endowment at Texas A&M University. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

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doi = "10.3390/metabo10100421",

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AU - Letsinger, Ayland C

AU - Menon, Rani

AU - Iyer, Anjushree R

AU - Vellers, Heather

AU - Granados, Jorge Z

AU - Jayaraman, Arul

AU - Lightfoot, J. Timothy

PY - 2020/10/20

Y1 - 2020/10/20

N2 - The gut metabolome offers insight for identifying the source of diet related pathology. As such, the purpose of this study was to characterize alterations of the gut metabolome in female and male C57BL/6J mice randomly assigned to a standard "chow" diet (CHOW) or a high fat/high sugar diet (HFHS; 45% fat and 20% fructose drinking solution) for nine weeks. Cecal metabolites were extracted and an untargeted analysis via LC-MS/MS was performed. Partial Least Sums Discriminate Analysis (PLS-DA) presented significant differences between the two diet groups in a sex-dependent manner. Mann-Whitney U-tests revealed 2443 and 1669 features to be significantly different between diet groups in the females and males, respectively. The majority of altered metabolites were depleted within the cecum of the HFHS fed mice. Metabolic pathways associated with galactose metabolism, leukotriene metabolism, and androgen and estrogen biosynthesis and metabolism were differentially altered with an HFHS diet b

AB - The gut metabolome offers insight for identifying the source of diet related pathology. As such, the purpose of this study was to characterize alterations of the gut metabolome in female and male C57BL/6J mice randomly assigned to a standard "chow" diet (CHOW) or a high fat/high sugar diet (HFHS; 45% fat and 20% fructose drinking solution) for nine weeks. Cecal metabolites were extracted and an untargeted analysis via LC-MS/MS was performed. Partial Least Sums Discriminate Analysis (PLS-DA) presented significant differences between the two diet groups in a sex-dependent manner. Mann-Whitney U-tests revealed 2443 and 1669 features to be significantly different between diet groups in the females and males, respectively. The majority of altered metabolites were depleted within the cecum of the HFHS fed mice. Metabolic pathways associated with galactose metabolism, leukotriene metabolism, and androgen and estrogen biosynthesis and metabolism were differentially altered with an HFHS diet b

KW - Androgen

KW - Cecum

KW - Estrogen

KW - Fructose

KW - Inflammation

KW - Metabolism

KW - Neurotransmitter

KW - Obesity

KW - Western diet

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JO - Metabolites

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ER -

A High Fat/High Sugar Diet Alters the Gastrointestinal Metabolome in a Sex Dependent Manner

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Keywords

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