TY - JOUR
T1 - A functional EDS1 ortholog is differentially regulated in powdery mildew resistant and susceptible grapevines and complements an Arabidopsis eds1 mutant
AU - Gao, Fei
AU - Shu, Xiaomei
AU - Ali, Mohammad Babar
AU - Howard, Susanne
AU - Li, Nan
AU - Winterhagen, Patrick
AU - Qiu, Wenping
AU - Gassmann, Walter
N1 - Funding Information:
Acknowledgments We thank Laszlo Kovacs for discussions and comments on the manuscript, and for providing mock-and MeSA-treated ‘Cabernet Sauvignon’ leaf tissue for EDL transcript quantiWca-tion. We thank Daniel Schachtman for providing cDNA libraries of PM-inoculated ‘Norton’ and ‘Cabernet Sauvignon’. This research was supported by Grants from the US Department of Agriculture Cooperative State Research, Education and Extension Service (2006-38901-03542 and 2008-38901-19367).
PY - 2010/4
Y1 - 2010/4
N2 - Vitis vinifera (grapevine) is the most economically important deciduous fruit crop, but cultivated grapevine varieties lack adequate innate immunity to a range of devastating diseases. To identify genetic resources for grapevine innate immunity and understand pathogen defense pathways in a woody perennial plant, we focus in this study on orthologs of the central Arabidopsis thaliana defense regulator ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1). The family of EDS1-like genes is expanded in grapevine, and members of this family were previously found to be constitutively upregulated in the resistant variety 'Norton' of the North American grapevine species Vitis aestivalis, while they were induced by Erysiphe necator, the causal agent of grapevine powdery mildew (PM), in the susceptible V. vinifera variety 'Cabernet Sauvignon'. Here, we determine the responsiveness of individual EDS1-like genes in grapevine to PM and salicylic acid, and find that EDS1-like paralogs are differentially regulated in 'Cabernet Sauvignon', while two are constitutively upregulated in 'Norton'. Sequencing of VvEDS1 and VaEDS1 cDNA and genomic clones revealed high conservation in the protein-encoding sequence and some divergence of the promoter sequence in the two grapevine varieties. Complementation of the Arabidopsiseds1-1 mutant showed that the EDS1-like gene with highest predicted amino acid sequence similarity to AtEDS1 from either grapevine varieties is a functional ortholog of AtEDS1. Together, our analyses show that differential susceptibility to PM is correlated with differences in EDS1 expression, not differences in EDS1 function, between resistant 'Norton' and susceptible 'Cabernet Sauvignon'.
AB - Vitis vinifera (grapevine) is the most economically important deciduous fruit crop, but cultivated grapevine varieties lack adequate innate immunity to a range of devastating diseases. To identify genetic resources for grapevine innate immunity and understand pathogen defense pathways in a woody perennial plant, we focus in this study on orthologs of the central Arabidopsis thaliana defense regulator ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1). The family of EDS1-like genes is expanded in grapevine, and members of this family were previously found to be constitutively upregulated in the resistant variety 'Norton' of the North American grapevine species Vitis aestivalis, while they were induced by Erysiphe necator, the causal agent of grapevine powdery mildew (PM), in the susceptible V. vinifera variety 'Cabernet Sauvignon'. Here, we determine the responsiveness of individual EDS1-like genes in grapevine to PM and salicylic acid, and find that EDS1-like paralogs are differentially regulated in 'Cabernet Sauvignon', while two are constitutively upregulated in 'Norton'. Sequencing of VvEDS1 and VaEDS1 cDNA and genomic clones revealed high conservation in the protein-encoding sequence and some divergence of the promoter sequence in the two grapevine varieties. Complementation of the Arabidopsiseds1-1 mutant showed that the EDS1-like gene with highest predicted amino acid sequence similarity to AtEDS1 from either grapevine varieties is a functional ortholog of AtEDS1. Together, our analyses show that differential susceptibility to PM is correlated with differences in EDS1 expression, not differences in EDS1 function, between resistant 'Norton' and susceptible 'Cabernet Sauvignon'.
KW - Arabidopsis
KW - Disease resistance
KW - Powdery mildew
KW - Vitis aestivalis
KW - Vitis vinifera
UR - http://www.scopus.com/inward/record.url?scp=77950535114&partnerID=8YFLogxK
U2 - 10.1007/s00425-010-1107-z
DO - 10.1007/s00425-010-1107-z
M3 - Article
C2 - 20145949
AN - SCOPUS:77950535114
SN - 0032-0935
VL - 231
SP - 1037
EP - 1047
JO - Planta
JF - Planta
IS - 5
ER -