A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker

Linda H. Malkas; Brittney Shea Herbert; Waleed Abdel-Aziz; Lacey E. Dobrolecki; Yang Liu; Beamon Agarwal; Derek Hoelz; Sunil Badve; Lauren Schnaper; Randy J. Arnold; Yehia Mechref; Milos V. Novotny; Patrick Loehrer; Robert J. Goulet; Robert J. Mickey

doi:10.1073/pnas.0604614103

A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker

Linda H. Malkas, Brittney Shea Herbert, Waleed Abdel-Aziz, Lacey E. Dobrolecki, Yang Liu, Beamon Agarwal, Derek Hoelz, Sunil Badve, Lauren Schnaper, Randy J. Arnold, Yehia Mechref, Milos V. Novotny, Patrick Loehrer, Robert J. Goulet, Robert J. Mickey

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104 Scopus citations

Abstract

Two isoforms of proliferating cell nuclear antigen (PCNA) have been observed in breast cancer cells. Commercially available antibodies to PCNA recognize both isoforms and, therefore, cannot differentiate between the PCNA isoforms in malignant and non-malignant breast epithelial cells and tissues. We have developed a unique antibody that specifically detects a PCNA isoform (caPCNA) associated with breast cancer epithelial cells grown in culture and breast-tumor tissues. Immunostaining studies using this antibody suggest that the caPCNA isoform may be useful as a marker of breast cancer and that the caPCNA-specific antibody could potentially serve as a highly effective detector of malignancy. We also report here that the caPCMA isoform functions in breast cancer-cell DNA replication and interacts with DNA polymerase S. Our studies indicate that the caPCMA isoform may be a previously uncharacterized detector of breast cancer.

Original language	English
Pages (from-to)	19472-19477
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	103
Issue number	51
DOIs	https://doi.org/10.1073/pnas.0604614103
State	Published - Dec 19 2006

Keywords

DNA replication
Genome stability
Mass spectrometry
Pathology
Posttranslational modification

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Malkas, L. H., Herbert, B. S., Abdel-Aziz, W., Dobrolecki, L. E., Liu, Y., Agarwal, B., Hoelz, D., Badve, S., Schnaper, L., Arnold, R. J., Mechref, Y., Novotny, M. V., Loehrer, P., Goulet, R. J., & Mickey, R. J. (2006). A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker. Proceedings of the National Academy of Sciences of the United States of America, 103(51), 19472-19477. https://doi.org/10.1073/pnas.0604614103

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title = "A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker",

abstract = "Two isoforms of proliferating cell nuclear antigen (PCNA) have been observed in breast cancer cells. Commercially available antibodies to PCNA recognize both isoforms and, therefore, cannot differentiate between the PCNA isoforms in malignant and non-malignant breast epithelial cells and tissues. We have developed a unique antibody that specifically detects a PCNA isoform (caPCNA) associated with breast cancer epithelial cells grown in culture and breast-tumor tissues. Immunostaining studies using this antibody suggest that the caPCNA isoform may be useful as a marker of breast cancer and that the caPCNA-specific antibody could potentially serve as a highly effective detector of malignancy. We also report here that the caPCMA isoform functions in breast cancer-cell DNA replication and interacts with DNA polymerase S. Our studies indicate that the caPCMA isoform may be a previously uncharacterized detector of breast cancer.",

keywords = "DNA replication, Genome stability, Mass spectrometry, Pathology, Posttranslational modification",

author = "Malkas, {Linda H.} and Herbert, {Brittney Shea} and Waleed Abdel-Aziz and Dobrolecki, {Lacey E.} and Yang Liu and Beamon Agarwal and Derek Hoelz and Sunil Badve and Lauren Schnaper and Arnold, {Randy J.} and Yehia Mechref and Novotny, {Milos V.} and Patrick Loehrer and Goulet, {Robert J.} and Mickey, {Robert J.}",

year = "2006",

month = dec,

day = "19",

doi = "10.1073/pnas.0604614103",

language = "English",

volume = "103",

pages = "19472--19477",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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Malkas, LH, Herbert, BS, Abdel-Aziz, W, Dobrolecki, LE, Liu, Y, Agarwal, B, Hoelz, D, Badve, S, Schnaper, L, Arnold, RJ, Mechref, Y, Novotny, MV, Loehrer, P, Goulet, RJ & Mickey, RJ 2006, 'A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker', Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 51, pp. 19472-19477. https://doi.org/10.1073/pnas.0604614103

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T1 - A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker

AU - Malkas, Linda H.

AU - Herbert, Brittney Shea

AU - Abdel-Aziz, Waleed

AU - Dobrolecki, Lacey E.

AU - Liu, Yang

AU - Agarwal, Beamon

AU - Hoelz, Derek

AU - Badve, Sunil

AU - Schnaper, Lauren

AU - Arnold, Randy J.

AU - Mechref, Yehia

AU - Novotny, Milos V.

AU - Loehrer, Patrick

AU - Goulet, Robert J.

AU - Mickey, Robert J.

PY - 2006/12/19

Y1 - 2006/12/19

N2 - Two isoforms of proliferating cell nuclear antigen (PCNA) have been observed in breast cancer cells. Commercially available antibodies to PCNA recognize both isoforms and, therefore, cannot differentiate between the PCNA isoforms in malignant and non-malignant breast epithelial cells and tissues. We have developed a unique antibody that specifically detects a PCNA isoform (caPCNA) associated with breast cancer epithelial cells grown in culture and breast-tumor tissues. Immunostaining studies using this antibody suggest that the caPCNA isoform may be useful as a marker of breast cancer and that the caPCNA-specific antibody could potentially serve as a highly effective detector of malignancy. We also report here that the caPCMA isoform functions in breast cancer-cell DNA replication and interacts with DNA polymerase S. Our studies indicate that the caPCMA isoform may be a previously uncharacterized detector of breast cancer.

AB - Two isoforms of proliferating cell nuclear antigen (PCNA) have been observed in breast cancer cells. Commercially available antibodies to PCNA recognize both isoforms and, therefore, cannot differentiate between the PCNA isoforms in malignant and non-malignant breast epithelial cells and tissues. We have developed a unique antibody that specifically detects a PCNA isoform (caPCNA) associated with breast cancer epithelial cells grown in culture and breast-tumor tissues. Immunostaining studies using this antibody suggest that the caPCNA isoform may be useful as a marker of breast cancer and that the caPCNA-specific antibody could potentially serve as a highly effective detector of malignancy. We also report here that the caPCMA isoform functions in breast cancer-cell DNA replication and interacts with DNA polymerase S. Our studies indicate that the caPCMA isoform may be a previously uncharacterized detector of breast cancer.

KW - DNA replication

KW - Genome stability

KW - Mass spectrometry

KW - Pathology

KW - Posttranslational modification

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 51

ER -

A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker

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